الفهرس 
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Abstract
Summary
Salivary gland tumors constitute an important area of oral and
maxillofacial pathology which may present significant diagnostic and
management challenges. Recent studies have begun to shed some light on the
molecular aberrations that govern the neoplastic transformation and
progression in SGTs.
This knowledge has the potential to translate into new therapies that
will specifically target the oncogenic events associated with the growth,
survival and spread of SGTs.
Survivin, an inhibitor of apoptosis has shown to be a significant
prognostic indicator in various human cancers.
This study was conducted to immunohistochemically investigate the
expression of VEGF and survivin proteins in different benign and malignant
salivary gland tumors and to correlate the expression of VEGF and survivin
in benign and malignant salivary gland tumors.
Forty formalin-fixed, paraffin embedded-specimens of different
benign and malignant salivary gland tumors were used in this study. Eight
cases were diagnosed as pleomorphic adenoma, twelve cases were diagnosed
as warthin’s tumor, five cases were diagnosed as mucoepidermoid
carcinoma, eight cases were diagnosed as adenoid cystic carcinoma
(Cylindroma) and seven cases diagnosed as adenocarcinoma not otherwise
specified.
Streptavidin-biotin immunoperoxidase staining system was used for
immune-detection where each lesion was stained with antibodies against
Summary
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VEGF and survivin proteins. For all cases, the area fraction of
immunopositivity for four different microscopic fields was measured. The
mean area fraction was then calculated and used for statistical analysis.
Immunohistochemical results of the present study revealed a higher
VEGF expression in malignant tumors when compared to benign tumors.
ANOVA test revealed significant differences for VEGF expression between
benign and malignant salivary gland, it shows that the mean for expression of
VEGF is highest in ACC & MEC and lowest in Warthin tumor.
Post Hoc test shows that there is a highly statistically significant
difference between Expression of VEGF in Pleomorphic Adenoma & (Warthin
Tumor, MEC & ACC), also there is a highly statistically significant difference
between Expression of VEGF in Warthin Tumor & (Pleomorphic Adenoma,
ACNOS, MEC & ACC), Moreover there is a highly statistically significant
difference between Expression of VEGF in ACNOS, Warthin Tumor, MEC &
ACC. So VEGF is a reliable marker in the diagnosis of the malignant salivary
gland tumors examined in the present study.
The expression of survivin protein was found to be greater in
malignant salivary gland tumors than in benign salivary gland tumors.
ANOVA test revealed significant differences for survivin expression between
benign and malignant salivary gland tumors examined in the present study, it
shows that mean for expression of Survivin is highest in ACC & MEC and
lowest in Pleomorphic Adenoma.
Post Hoc test shows shows that there is a highly statistically
significant difference between Expression of Survivin in Pleomorphic
Adenoma & (Warthin Tumor, ACNOS, MEC & ACC), also there is a highly
statistically significant difference between Expression of Survivin in Warthin
Tumor & (Pleomorphic Adenoma, MEC & ACC), Moreover there is a highly
statistically significant difference between expression of Survivin in ACNOS,
Pleomorphic Adenoma & ACC.
Independent sample t-test shows that there is no statistically
significant difference between Expression of Survivin & VEGF in Benign
and malignant salivary gland tumors.
Pearson correlation indicates that there is no Correlation between
Expression of Survivin and VEGF in Benign Salivary Gland Tumors and that
there is a very weak statistically significant Correlation between Expression
of Survivin and VEGF in Malignant Salivary Gland Tumors.