الفهرس 
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Abstract
This study aimed to determine the effects of supplementation of both Zn and Se on the activities of some antioxidant enzymes as well as the effect of these metals on the expression of Metallothionein (Mt) gene. This study was carried out on fifty male Wister albino rats weighted 200 gram. Ten rats were injected with saline and served as the first group. The remaining 40 rats were injected intraperitoneally with streptozotocin (STZ) 50 mg/kg body weight for experimental induction of diabetes and used as second, third, fourth and fifth groups each of ten rats. The second group was left untreated, the third group was treated with insulin 1 IU/100 g B.Wt, the forth group was treated with zinc supplied in diet at a dose of 180 mg zinc chloride/kg diet, while the fifth group was administered selinium (as sodium selenite) at a dose of 1.27 mg/kg B.Wt orally by gavaging. The obtained results revealed that the whole blood and hepatic SOD activity and GSH levels were significantly decreased in diabetic group compared to control group. Zn and Se supplementation to diabetic rats significantly restored SOD and GSH levels. In addition, serum and hepatic MDA levels were significantly higher in diabetic rats than controls. Insulin, Zn, and Se treatment led to a significant decrease in both serum and liver MDA concentrations and restoring normal levels. The hepatic MT gene expression was significantly lowered in STZ-diabetic rats. Such a decrease was reversed by different treatments with Zn causing the maximum induction. from the obtained results, it was concluded that both Zn and Se could play a role in ameliorating oxidative stress and controlling hyperglycemia in diabetes. This effect could be in part due to their stimulatory effect on MT gene expression.