الفهرس 
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Abstract
I
n this study, we were concerned To evaluate foxp3 mRNa level in mononuclear cells in children with Acute thrombocytopenic purpura and the relationship between foxp3mRNA level and platelet count response to therapy will be also studied, that was carried out on 15 patients at the the Pediatric Hematology unit, from Children’s Hospital, Ain Shames University during the period from December 2012 to February 2013.
Clinical evaluation of patients with assessment of the disease activity by complete blood count and Foxp3 mRNA level gene expression by real-time polymerase chain reaction in mono nuclear cells.
The present study revealed that 13 patients gave clinical response, 2 patients failed to give clinical response.
There was no significant difference in the level of Foxp3 gene expression between the studied patients and healthy subjects.
There was significant correlation between the level of Foxp3 gene expression and ages and the age at onset of ITP of the studied patients, on the other hand there was no significant correlation between Foxp3 gene expression and ITP duration, PLT count and number of relapses.
The level of Foxp3 gene expression didnot correlate significantly with the platelets count at six month of the diagnosis.There was no significant correlation between the level of foxp3 gene expression and the cumulative dose of steroids and IVIG.
In conclusion, our study did not show significant difference in the level of FOXP3 expression in mononuclear cells among patients with acute ITP as compared to healthy subjects. Also, level of FOXP3 expression did not affect the presentation of acute ITP nor affected by the treatment regimen given (Corticosteroids and I.VIG). This study has limitation in terms of lack of assessment of number and function of nTregs among these patients which interfere with demonstration of the role of nTregs in acute I.T.P. Further wider scale studies are needed to evaluate the number and function of nTregs in acute ITP giving more clear understanding of its disorders in acute ITP and hence the possible therapeutics options targeting nTregs in those patients.