الفهرس 
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Abstract
This study was aimed to detect some virulence genes of E. coli isolated from diarrheic dogs and cats. from 90 rectal swabs collected from diarrheic pets 33 E. coli isolates were recoverd with a percentage of (36.6 %), 12 were haemolytic (36.3%).E. coli was isolated at highest percentage from diarrheic dogs and cats at young age ranged from 1-6 months.E. coli isolated from diarrheic dogs gave alpha, beta and gamma hemolysis at percentage of 23.07%, 11.53% and 65.38% respectively. Moreover, E. coli isolated from diarrheic cats gave alpha, beta and gamma hemolysis at percentage of 28.57%, 14.28% and 57.14% respectively. The results of E. coli serotyping were recorded as 2 isolates (16.6%) were + poly 2 (O:127: K 63), one isolate (8%) was +poly 3 (O:164 K -) , one isolate (8%) was + poly 1 (O26 :K 60) and 8 isolates (66.6%) positive poly one .The results of intimin (eaeA) gene detection revealed that the gene was detected in 4 isolates (33.3%) three from dogs (one of serotype O:26: K60 , two serotyped as positive poly one), and one from a cat serotyped as positive poly one.The results of Bfb gene detection revealed that the gene could not detected in 4 examined isolates which were positive for eaeA gene. EPEC strains isolated from dogs and cats were atypical EPEC (only contained the eaeA gene and negative for Bfp gene).The results of Shiga toxin genes detection in 12 pathogenic E. coli isolated from diarrheic dogs and cats revealed that the gene could not detected in all examined isolates.The results of Enterotoxin genes detection in 12 pathogenic E. coli isolated from diarrheic dogs and cats revealed that the gene could not detected in all examined isolates.The results of antibiotic sensitivity showed high resistance for Cephalexin, Ampicillin sulbactam and Gentamycin that are commonly used in veterinary practice. And a high sensitivity for Amikacin and Ofloxacin was observed. The presence of antimicrobial resistance among strains explain the need for strict measures to regulate the use of antimicrobials and denoting the relevance of surveys of E. coli as a potential zoonotic cause of human diarrhea and emphasizes the epidemiological role of pets as reservoirs of highly pathotypes.This study was aimed to detect some virulence genes of E. coli isolated from diarrheic dogs and cats. from 90 rectal swabs collected from diarrheic pets 33 E. coli isolates were recoverd with a percentage of (36.6 %), 12 were haemolytic (36.3%).E. coli was isolated at highest percentage from diarrheic dogs and cats at young age ranged from 1-6 months.E. coli isolated from diarrheic dogs gave alpha, beta and gamma hemolysis at percentage of 23.07%, 11.53% and 65.38% respectively. Moreover, E. coli isolated from diarrheic cats gave alpha, beta and gamma hemolysis at percentage of 28.57%, 14.28% and 57.14% respectively. The results of E. coli serotyping were recorded as 2 isolates (16.6%) were + poly 2 (O:127: K 63), one isolate (8%) was +poly 3 (O:164 K -) , one isolate (8%) was + poly 1 (O26 :K 60) and 8 isolates (66.6%) positive poly one .The results of intimin (eaeA) gene detection revealed that the gene was detected in 4 isolates (33.3%) three from dogs (one of serotype O:26: K60 , two serotyped as positive poly one), and one from a cat serotyped as positive poly one.The results of Bfb gene detection revealed that the gene could not detected in 4 examined isolates which were positive for eaeA gene.EPEC strains isolated from dogs and cats were atypical EPEC (only contained the eaeA gene and negative for Bfp gene).The results of Shiga toxin genes detection in 12 pathogenic E. coli isolated from diarrheic dogs and cats revealed that the gene could not detected in all examined isolates.The results of Enterotoxin genes detection in 12 pathogenic E. coli isolated from diarrheic dogs and cats revealed that the gene could not detected in all examined isolates.The results of antibiotic sensitivity showed high resistance for Cephalexin, Ampicillin sulbactam and Gentamycin that are commonly used in veterinary practice. And a high sensitivity for Amikacin and Ofloxacin was observed. The presence of antimicrobial resistance among strains explain the need for strict measures to regulate the use of antimicrobials and denoting the relevance of surveys of E. coli as a potential zoonotic cause of human diarrhea and emphasizes the epidemiological role of pets as reservoirs of highly pathotypes.