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Abstract The present work aims to study the ecological studies on mite species associated with maize and wheat crops at Sharkia province, control studies in laboratory and field using certain biocides against phytophagous and predetory mites as well as some entomofungal isolates of biological control against phytophagous and finally, biochemical analysis of maize leaves in relation to biocides treatment and population of Tetranychus urticae. The results identified seven mite species belonging to six families in the total samples collected from maize plants and three species collected from wheat plants. These species were classified according to their feeding habits to three major groups i.e., phytophagous mites (2 species), predaceous mites (3 species) and uncertain feeding behavior mites (2 species). Tetranychus urticae from the phytophagous and Amblyseius enab and Agistemus exsertus from the predators were most dominant species associated with maize plant in all districts of Sharkia Governorate. Values of absolute and relative frequency of occurrence for mite species were calculated. In addition, species affected significantly with temperature where the mean number of T. urticae, A. enab and A. exsertus were the highest in accordance with the highest temperature and less humidity values. Data recorded six species belonging to three families isolated from maize crop soil in Sharkia Governorate. The efficiency of tested biocides against T. urticae was tested experimentally. It can be concluded that Vertimec was the most toxic biocide with LC50 of 0.008 ppm compared to the other tested ones. Considering the toxicity index percentage at LC50, the most potent toxic biocide was Vertimec with toxicity index 100%, followed by Tracer and Bioranza recording 0.09% and 0.023%, respectively, while the Dippel 2X had the least toxicity recording 0.00217%. Data showed that |