الفهرس 
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Abstract
Remineralization of hard dental tissues is thought to be a tool that could close the gap between prevention and surgical procedures in clinical dentistry. The aim of this study is to evaluate in vitro, the effect of ozone gas using different remineralizing material on non-cavitated caries-like lesions in permanent teeth.
Sixty sound premolars extracted for orthodontic reasons. The premolars were free from any clinical sign of decalcification or enamel defects indicating score 0 according to ICDAS-II and DIAGNOdent reading between 0-9.
Each tooth was sectioned into two halves, one was treated and the other served as control. The tooth specimens were randomly divided into three groups (I, II, and III), each group was subdivided into two subgroups (n=20), experimental [I(a), II(a), and III(a)] and control [I(b), II(b), and III(b)]. All specimens were immersed in demineralizing solution for 72 hours at room temperature, to create an early enamel lesion.
Then subgroup I(a) was treated with ozone gas followed by fluoridated toothpaste, subgroup II(a) was treated with ozone gas followed by HealOzone remineralizing solution and oral hygiene patient kit, and subgroup III(a) was treated with ozone gas followed by fluoride varnish and fluoridated toothpaste. Subgroup I(b), II(b), and III(b) left untreated. All the specimens were kept in artificial saliva.
After 4 weeks the elemental distribution on enamel surface was measured by SEM-EDX. There were significant increase in the mean Ca/P in the 3 subgroups [I(a), II(a) and III(a)] when comparing with their controls indicating surface lesion remineralization.
The mean Zn values were significantly increased in subgroup II(a) compared with its control, when using ozone followed by HealOzone remineralizing solution and patient kit. On the other hand mean Zn values were significantly reduced in subgroups I(a) and III(a).
When comparing the 3 test subgroups there was no significant difference regarding Ca/P ratio. But according to Zn there was significant difference favoring subgroup II(a) which is treated with ozone followed by HealOzone remineralizing solution and patient kit.
The results of this in vitro study showed that the use of ozone followed by remineralizing material can remineralize subsurface enamel lesions in permanent teeth.