الفهرس 
DIABETES MELLITUSOnly 14 pages are availabe for public view
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Abstract
Telomeres are DNA-protein complexes at the ends of eukaryotic chromosomes that are essential for their function and genomic stability.
The telomeric complexes prevent the ends of linear chromosomes from being recognized as broken ends, which would otherwise elicit inappropriate DNA damage responses with potentially deleterious consequences.
Telomerase, a specialized reverse transcriptase that extends the single-stranded G-rich 3’ protruding ends of chromosomes, stabilizes telomere length in Germ line cells and regenerative tissues as well as in tumor cellsTelomerase, the non-coding DNA sequences capping the end of eukaryotic chromosomes consist of tandem TTAGGG repeats, forming a highly organized structure which is stabilized by several telomeric proteins. This structure protects chromosomes from being recognized as DNA breaks domains, thus conferring stability to the whole genome.
Diabetes mellitus (D.M) is a syndrome of disordered metabolism. Usually due to a combination of hereditary and environmental causes, resulting in abnormally high blood sugar levels (hyperglycemia).
Diabetes mellitus (D.M) is associated with damage to target organs and premature aging and telomeres serve as a mitotic clock and biological
marker of senescence.
The current study aimed to evaluate telomerase activity in type 1 diabetic patients without micro- or macroangiopathic complications.
This work was performed on 20 patients with type 1 diabetes mellitus without micro or macroangiopathic complications and 20 apparently healthy volunteers.
All patients were subjected to a thorough history and clinical examination with stress on the following: (type of diabetes, mode of therapy, microangiopathy, macroangiopathy, duration of diabetes). All subjects (patients & control) were investigated by the following laboratory investigations: FBS, PPBS, HbA1C, lipid profile, creatinine, microalbumin and measuring telomerase activity in mononuclear cells extract using Telomerase PCR ELISA PLUS kit by Photometric Enzyme Immunoassay (TRAP).
The results of the present work showed that:
• Significant decrease in telomerase activity in diabetics compared to controls
• Significant negative correlation between telomerase activity and each of HBA1C level, , FBS and PPBS in the case group.
• Significant increase in LDL level in diabetic patients compared to control group.
• Significant decrease in HDL level in diabetic patients compared to control group.
• Significant increase in TG level in diabetic patients compared to control group.
• Significant increase in TCH level in diabetic patients compared to control group.