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العنوان
Utilization of proteomic, temperature tolerance and metabolomic analysis to select gene candidates for controlling Asian Citrus Psyllid, Diaphorina citri Kuwayama, using RNA interference /
المؤلف
El-Shesheny, Ibrahim Abdelhak Mohamed.
هيئة الاعداد
باحث / ابراهيم عبد الحق محمد الشيشينى
مشرف / ابراهيم سعيد الهوارى
مناقش / ابراهيم ابراهيم مصباح
مناقش / نبيل قلينى
الموضوع
Plant Protection.
تاريخ النشر
2015.
عدد الصفحات
p 173. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم الحشرات
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة طنطا - كلية الزراعة - Plant Protection
الفهرس
Only 14 pages are availabe for public view

from 203

from 203

Abstract

Huanglongbing (HLB), or citrus greening disease, seriously threatens the citrus industry worldwide. Its management critically depends on the control of the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), the vector of Candidatus Liberibacter asiaticus bacteria (CLas), the putative causal agent of HLB. For controlling this insect, this study was carried out in the Citrus Research and Education Center, institute of food and Agricultural sciences, University of Florida, USA. In this study, we investigated developmental proteomic changes of ACP. A proteomic comparative study between the 5th nymphal instar and the adult stage was carried out. The functions of the identified proteins were reviewed. A critical protein named NDPK which is a product of an important gene named abnormal wing disc (awd) was targeted using RNAi application. The role of awd in ACP development and wing formation was demonstrated. The Interference of awd transcription by micro-application of dsRNA to ACP nymphs was investigated. Since awd gene was found to be implicated also in temperature tolerance, the effect of temperature on the survival, energy metabolism and gene expression dynamic was studied.