الفهرس 
Introduction:Only 14 pages are availabe for public view
 |  | from | 209 |  |  |
| | | from | 209 | | |
Abstract
Stroke is the third most common cause of death in most industrialized countries after cardiovascular disease and cancer, and its incidence is expected to rise with the projected increase in the number of the aging population. Cerebral ischaemia reperfusion (I/R) triggers a complex series of biochemical events that ultimately lead to neurodegeneration and cell death.
The present work was performed to compare between the effects of different drug classes used in treatment of stroke on some biochemical parameters ocurrs during experimental brain I/R in rats. Extension study was performed to give an insight about the role of PPAR-γ agonist by using rosiglitazone on the neuroprotection effect of vincamine, vinpocetine, cinnarizine, or pyritinol that used in stroke. Studying the possible beneficial interaction between two drugs used in stroke was also investigated.
Male Wistar rats were divided into 17 groups. The first group was subjected to 1hr of brain ischaemia, then left 2hr for reperfusion and this group is considered as ischaemic group. The second group was the treatment groups, where 15 group of rats divided randomly, where the animals subjected to brain ischaemia for 1hr followed by drug administration and reperfusion for 2hr. five groups were received single oral dose of freshly prepared aqueous suspension of vincamine (6 mg/kg body weight), vinpocetine (3 mg/kg body weight), cinnarizine (7 mg/kg body weight), pyritinol (55 mg/kg body weight), and rosiglitazone (0.5 mg/kg body weight). Four groups were received a daily single oral dose of a combination of freshly prepared aqueous suspension of rosiglitazone with vincamine, vinpocetine, cinnarizine, or pyritinol respectively. Furthure, six groups of animals were received half the previous single oral dose of freshly prepared aqueous suspension of combination of vincamine, vinpocetine, cinnarizine, or pyritinol. The sham-operated group underwent identical surgical manipulation except that occlusion of the carotid arteries.
Rats were anaesthetized with i.p. injection of urethane (1.3 g/ kg) then brain I/R was induced by clamping of both common carotid arteries for 1hr followed by declamping and reperfusion for 2hr.
At the end of reperfusion period animals were scarified by decapitation and the whole brain of each animal was isolated and blood samples were also collected. The following biochemical parameters were measured.
1. Brain superoxide dismutase activity (SOD).
2. Brain glutathione content (GSH).
3. Serum catalase activity (CAT).
4. Serum NO levels.
5. Brain malondialdhyde level (MDA).
6. Serum lactate dehydrogenase activity (LDH).
7. Serum glucose-6-phosphate dehydrogenase activity (G6PDH.