الفهرس 
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Abstract
The work presented in the thesis represents plant tissue culture of two medicinal aromatic plants (Ocimum basilicum L. and Pimpinella anisum L.) and bilological screening of cytotoxicity, antioxidant, and immunostimulant activities of their extracted oils.
The thesis consists of three parts:
Part I
Plant tissue culture of Ocimum basilicum L. and Pimpinella anisum L. and examination of calli for cytodifferentiation and organogenesis.
Since plant tissue culture systems represent a potential renewable source of valuable medicinal compounds which cannot be produced by microbial cells or chemical synthesis. So, we applied this technology on two important aromatic plants which can be widely used for medicinal and non- medicinal purposes all over the world. The formed calli and tissues from culture system indicates the formation of different organs and xylem tissue which refers to a high degree of cytodifferentiation and these tissues were microscopically examined.
Part II
A. Extraction of essential oil from Ocimum basilicum L. and Pimpinella anisum L. calli and assessment of the yield.
B. Gas chromatography- Mass spectral analysis (GC/MS) of the extracted oils.
Since the main purpose of our tissue culture work was the production of secondary metabolites of interest. So, essential oils for both cultures were extracted and their yield of essential oil was obtained at different time intervals of the culture time. Gas chromatography- Mass spectral analysis was essential for identification of components in the extracted oils.
Part III
Chapter 1- Cytotoxcic activity of essential oil of Ocimum basilicum L. and Pimpinella anisum L. on normal peripheral blood cells (PBMCs), hepatocellular carcinoma (HepG-2) and colorectal adenocarcinoma (Caco-2).