الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Pseudomonas aeruginosa is an opportunistic human pathogen and its infection is life-threatening for immuno-compromised patients, P. aeruginosa has the ability to form biofilm and one of the best-known of these biofilm-specific properties is antibiotic resistance that can be up to 1000-fold greater than planktonic cells. An antibiotic resistance mechanism in P. aeruginosa is the transport of antimicrobials via efflux pump systems, especially those of the resistance-nodulation-cell division (RND). A novel efflux pump in P. aeruginosa was identified to be important for biofilm-specific resistance to a subset of antibiotics.
Biofilm production was detected by tube method, antibiotic susceptibility test was done by broth microdilution method in 96-well micotitre plate, expression of efflux pump gene PA1874 was measured by quantitative real time PCR (SYBR Green).
We conclude that biofilm form is the most resistant form of P. aeruginosa and this biofilm specific resistance was associated with the highest degree of expression the novel efflux pump gene PA1874 .
Key words: Pseudomonas aeruginosa, Biofilm, Efflux pump, PA1874 gene.