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Abstract Evauation of newly formalized pollen supplemental diets through determining the consumption rate of each diet testing the impact of these diets on honey bee colonies measuring the increase in sealed working area and hypopharyngeal gland development. Honey bees are hymenopterans defined as a subset of bees in the genus Apis, primarily distinguished by the production and storage of honey and the construction of perennial, colonial wax nests. Honeybees are the only extant members of the tribe Apini, all in the genus Apis. Only members of the genus Apis are true Honeybees, although some other types of related bees produce and store honey for minor honey productionHoney bees study has gained a special interest due to their importance for the human being as well as maintaining the agricultural biodiversity. Losing them could affect not only dietary staples such as apples, broccoli, strawberries, nuts, asparagus, blueberries and cucumbers, but may threaten our beef and dairy industries if alfalfa is not available for feed.Arguably, the real value of honey bees is more appropriately a deeply layered assessment of quality in life. Most calories consumed by humans are from crops that do not depend on insect pollination (corn, wheat, etc.), but as countries develop, higher quality foods are desired which are often insect pollinated (Wilson, 2011).Rearing honey bee queens is one of the most interesting and rewarding aspects of beekeeping. It is a topic that has received a great deal of attention and consideration through the years. It provides a means to maintain young, vigorous queens in colonies and is the foundation of good colony management to ensure giving maximum egg laying as well as minimum swarming tendency. This also enables a degree of selection for desirable and good colony characteristics (Laidlaw, 1979).As royal jelly is always fed directly to the queen or the larvae as it is secreted; it is not stored. This is why it has not been a traditional beekeeping product. where the only situation in which harvesting becomes feasible is during queen rearing, when the larvae destined to become queen bees are supplied with an overabundance of royal jelly. Thus queen rearing is crucial for royal jelly production for business purposes (Krell, 1996). Here in the Southern of Egypt, specifically Qena through Aswan, the only way for obtaining the queens is by importing them from those Egyptian cities situated in northern part. Consequently, it is significantly important to not only abridge the difficulty of travelling efforts, time, and risk, but finding an officially accredited alternative source as well; to provide dependable advices and recommendations for the uprising young beekeepers. So that, we are going to provide them with their need through theproposed work that focuses on searching a new method for queen rearing mainly. Aim of Work: • Evaluation of newly formalized pollen supplemental diets, through determining the consumption rate of each diet. • Testing the impact of these diets on honey bee colonies; measuring the increase in sealed workers brood area and hypopharyngeal gland development• Assessment of the acceptance percentage of the grafted larvae into the colony in the Apiary of Faculty of Agriculture, South Valley University. • Evolution of new method for a successful mass rearing of the honey bee queens. • Evaluation of the queens produced by the new method along with their offspring; through comparing them with queens reared by other well known methods |