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العنوان
Detection Of Some Enteric Pathogens In Meat =
المؤلف
Khaled, Kamal Antar Kamal.
هيئة الاعداد
باحث / كمال عنتر كمال خالد
مشرف / ابراهيم عبد التواب سماحة
مشرف / محمد السيد عبد اللطيف نصير
مناقش / نادر يحيي مصطفي
مناقش / محمد محمد ابراهيم موسي
الموضوع
Hygiene.
تاريخ النشر
2014.
عدد الصفحات
70 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
30/12/2014
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - الرقابة الصحية علي اللحوم
الفهرس
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Abstract

A total of 100 random samples of meat represented by cattle and buffaloes (50 of each) were collected from different butcher’s shops at Al-Bohiera Province. The samples were subjected to total Enterobacteriaceae count, total coliforms count and determine the frequency of isolation of members of family Enterobacteriaceae in addition to serological identification of E. coli and Salmonella isolates.The obtained results revealed that:Enterobacteriaceae were detected on 100% of the samples. The mean value of total Enterobacteriaceae count of examined meat samples of cattle were ranged from 2.6× 103 to 3.6× 104 with a mean value 1.4× 104 ± 1.4× 103, while the total Enterobacteriaceae count of examined meat samples of buffaloes were ranged from 2.9× 103 to 7.3× 104 cfu/g with a mean value 2.7× 104 ± 3.3× 103 cfu/g There is a significant difference between the mean values of total Enterobacteriaceae count of cattle and buffaloes (P < 0.05).T-test of total Enterobacteriaceae counts between cattle and buffaloes was 4.21 significant at (P < 0.01).Coliforms were detected on 100% of the samples.The mean value of total coliforms count cfu/g of cattle meat ranged from 1.6 × 103 to 2.8 × 104, with a mean value 1.0 × 104 ± 1.4 × 103 while the total coliforms count cfu/g of buffalo meat ranged from 2.1 × 103 to 3.6 × 104 with a mean value 1.8 × 104 ± 1.8 × 103. There is a significant difference between the mean values of total coliforms counts of cattle and buffaloes (P < 0.05). T-test of total coliforms count between cattle and buffaloes meat was 4.21 significant at (P <0.05). Identification of members of Enterobacteriaceae on cattle meat samples revealed the isolation of:E. coli was the highest isolated bacteria (32%) followed by K. pneumonia Subspecies Ozanea (12%). There were 16 biochemically identified E. coli isolates; O55 K59, O111 K58, O119 K96, O119 K69, O125 K70, O126 K71 and O127 K63 were isolated at the percentages of 4, 4, 4, 8, 4, 4, and 4%, respectively. There were two biochemically identified Salmonella isolates (4%); one isolate was serotyped as Salm. Enteritidis while the other one was serotyped as Salm. Typhi. Other enteric bacteria including; E. aerogenes, E. intermedium, E. gergoviae, C. amanlonticus, C. freundii, S. marcescems, S. Ficaria, S. Fonticola, S. liquefaciens, S. rubidaea, E. hoshinae, P. alcalifaciens, P. stuartii and P. mirabilis. Identification of members of Enterobacteriaceae on meat samples of buffaloes revealed the isolation of: E. coli was the highest isolated bacteria (40%) followed by K. pneumonia Subspecies Ozanea (16%).There were 20 E. coli isolates were identified biochemically from examined meat samples of buffaloes and serotyped as O55 K59, O111 K58, O114 K96, O119 K69, O125 K70 and O126 K7 at the percentages of 2, 6, 4, 10, 6 and12%, respectively. There were 4 Salmonella isolates were recovered where Salm. Enteritidis (2), Salm. Paratyphi (1) and Salm. Typhi (1). Other enteric bacteria including; E. aerogenes, E. intermedium, E. gergoviae, C. amanlonticus, C. freundii, S. marcescems, S. Ficaria, S. Fonticola, S. liquefaciens, S. rubidaea, E. hoshinae, P. alcalifaciens, P. stuartii and P. mirabilis could be isolated and identified at different percentagesOn the basis of the previously mentioned results, Enterobacteriaceae still constitutes common contaminants of meat of cattle and buffaloes and its presence in meat is very important due to its public health significance, therefore; good hygienic measures must be applied to obtain safe and wholesome meat.