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Abstract A total of 80 human sound molars were used to evaluate the immediate and delayed sealing ability of Single Bond Universal (3M, Germany) in terms of microleakage in respect to the used bonding strategies and different dentin surface states. Cavities in occlusal dentin were prepared and restored with the adhesive, followed by the application of Filtek Z250XT composite material (3M, Germany). After finishing and polishing procedures, the specimens’ microleakage assessment was performed with tracing Caries Indicator dye (Ultradent, USA) by its tracing under stereomicroscope and linear microleakage calculation by custom designed MATLAB® program. The statistical analysis showed significant increase in microleakage after 1 year of water storage of all specimens. It also showed that in the immediate specimens the self-etch mode was better than etch-and-rinse when used over moist dentin. While the etch-and-rinse mode was better than the self-etch mode when used over dry dentin. There was no significant difference between both bonding protocols. Within the limitations of this study, it can be concluded that: 1. The tested adhesive can be used in both etch-and-rinse and self-etching modes with the same efficiency, taking into consideration the dentin surface condition. 2. The long term water storage of the tested adhesive had negative effect on its sealing ability. 3. Chemical composition might influence the microleakage behavior of the tested adhesive by time. |