الفهرس 
Glass ionomerOnly 14 pages are availabe for public view
 |  | from | 127 |  |  |
| | | from | 127 | | |
Abstract
The aim of this study was to nvestigate the genotoxic and cytotoxic effects of commercially available conventional glass ionomer, resin modified glassionomer, flowable nanocomposite and low shrinkage silorane based composite on normal cultured human lymphocytes.
A total of 40 specimens of tested filling materials were used, samples were divided into two main groups. One for glass ionomer restorative material with its two subgroups, conventional (Ketac TM fil Plus / 3M ESPE) and resin modified glass ionomer (Photac TM fil / 3M ESPE). The other group was for the resin composite restorative material with its two subgroups, nano composite (Filtek TM Z350 XT /3M ESPE) and low shrinkage silorane composite (Filtek TM P90/ 3M ESPE).
Each specimen was prepared according to the manufacturer’s instructions and inserted aseptically into prefabricated moulds as disc of 8mm diameter and 2mm thickness, with surface area of 1.5 cm2.
Each sub group of specimens was sterilized in an autoclave in a separate sterilization packet as aseptic conditions are mandatory for cells growth in the next steps of cell culture.
Eluates of each material were prepared 24 hours after setting by placing each specimen in 2ml of extraction medium in microcentrifuge tube, extraction medium used was cell culture medium (RPMI 1640); with 100 IU/ml penicillin and 100 ug/ml streptomycin, All microcentrifuge tubes of eluates were incubated for 72 h at 37 cº in CO2 incubator.
We took 20 ml of venous blood using disposable sterile syringes from each individual (5ml for each experiment), Sampleswere placed immediately after being taken in heparinized blood vaccutainers and kept in refrigerator.
Eluate of each subgroup was tested on lymphocyte cell culture of each donor, and each test was repeated 3 times.
Cell cultures were harvested; cells were spread on freezed slides, stained by geimsa stain and examined under light microscope.
Chromosomal aberrations (CAs) assay test was done to investigate both genotoxic and cytotoxic effects.
The results of this study showed that conventional glass ionomer (Ketac TM fil Plus) and silorane based composite (Filtek TM P90) showed no deviation from negative control and showed no chromosomal aberrations, which indicates lack of toxicity of these materials.
Resin modified glass ionomer (Photac TM fil) and flowable nano composite (Filtek TM Z350 XT) showed increase in frequencies of chromosomal aberrations, which indicates genotoxicity and cytotoxicity of these materials.