الفهرس | Only 14 pages are availabe for public view |
Abstract Nicotine is a combination of pyridine and methyl pyrolidine, C10H14N2. This study was aimed to investigate the biochemical and histological effects of nicotine on testis of adult rats. Nicotine caused a significant malfunction (P < 0.05) in the mean values of serum sexual hormones, testosterone, ABP, FSH and LH concentration in the testis when compared with the control. It is concluded that nicotine caused decrease of testosterone and increase of ABP, FSH and LH concentrations in the serum. Also it causes histological changes in the testis and theses changes were dose dependndant. A total of adult 30 male rats weighing from (250-300) gm were divided into three groups: Group 1 (n=10), the control group was given normal saline1m subcutaneous pear day for 8 weak ; group 2 (n=10), was given nicotine at low dose ”0.25mg/kg” body weight;/ day/8weak, group 3 (n=10), was given nicotine at high dose 0.5mg/kg” body weight. After sacrifice, blood samples were collected in tubes, separated the serum for determination of testosterone, androgen binding protein (ABP), FSH and L.H. The results showed that serum testosterone levels were significantly decreased in both treated rats groups, low and high dose, compared with control animals (P < 0.05). on the other hand, serum androgen binding protein (ABP), follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels were significantly increased in both treated rats groups, low and high dose, compared with control animals (P < 0.05). Histological results revealed destruction changes in rates testis of low dose nicotine-treated group and this changes were increased with high dose –treated group. this study aimed to determining the biochemical effect of nicotine on male testis by evaluating, serum concentration of testosterone, androgen binding protein (ABP), FSH and LH. So . Nicotine should therefore be taken with caution to avoid its harmful effects on testis. |