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العنوان
Development of molecular methods of bacterial identification in food products by means of genomic and proteomic tools /
المؤلف
Elsayed, Mohamed Ezzat Ahmed.
هيئة الاعداد
باحث / Mohamed Ezzat Ahmed Elsayed
مشرف / Ibrahim Hassan I. Amer
مشرف / Magdy Sharaf Elsayed
مشرف / Madiha Abd-Allah Ayoub
مشرف / Jorge Barros Velazquez
الموضوع
Milk Hygiene.
تاريخ النشر
2014.
عدد الصفحات
264 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الزقازيق - كلية الهندسة - Department of Food Control / Milk Hygiene
الفهرس
Only 14 pages are availabe for public view

from 273

from 273

Abstract

Milk and chesses of ovine and caprine origins constitute an important category of human due to their high nutritive values and interesting organoleptic characters in the case of ovine and caprine chesses. Unfortunately, they can serve as good mediums for the growth of many undesirable microorganisms of spoilage or even pathogenic characters. In the case of raw milk, several bacterial species incriminated in causing mastitis in dairy animals, milk spoilage or even FP can be harbored. Likewise, consumption of chesses has reported to cause FP in human consumers as a result of improper production.
For the impact of microorganisms in dairy sector as well as public health issues, both Food sector and dairy sector safeties are greatly dependant on basis of microbial identification and differentiation. Historically, several morphologic and phenotypic based methods were used to perform these tasks. However, they are unsuitable nowadays due to consumption of longer time, huge amount of materials and labor and on the other hand, lowered diagnostic accuracy in comparison to the recent molecular approaches. As a consequence, various molecular methods have been applied in diagnostic laboratories to overcome the disadvantages obtained within traditional diagnostic tools. For this, current study was conducted to develop and assess some genomic (16 S rRNA GS, 23S rRNA GS, gyrA GS, gyrB GS and tuf GS) and proteomic tools (MALDI-TOF MS fingerprinting) for detection, diagnosis and taxonomical classification purposes for different microorganisms implicated in dairy safety and public health sectors. Also, the work has compared use of such molecular tools.