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العنوان
Evaluation of phenotypic and genotypic methods for identification of some dermatophytes isolated from human, animal and soil /
المؤلف
Younes, Ahmed Ismail Ahmed Ali.
هيئة الاعداد
باحث / Ahmed Ismail Ahmed Ali Younes
مشرف / Mohamed Taha Mahmoud
مشرف / Sabry Ismaeil Essa
مشرف / Sabry Ismaeil Essa
الموضوع
Veterinary Immunology. Dermatophytes- isolation & purification. Soil.
تاريخ النشر
2014.
عدد الصفحات
178 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Bacteriolog, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

from 202

from 202

Abstract

Dermatophytes are the main cause of superficial mycoses. These fungi have the capacity to invade keratinized tissue of humans or animals to produce dermatophytosis (ringworm) that are generally restricted to the corneocytes of the skin, hair and nail.
While identification of dermatophytes is usually depending on conventional methods based on detection of phenotypic characteristics, last few years have proven that genotypic methods are more rapid and accurate.
The present work was done to study methods of identification of dermatophytes isolated from human, animal and soil by conventional and genotypic methods.
Fifty five skin scrapings and hair from human dermatophytosis, 15 from animal dermatophytosis as well as 5 samples of soil were subjected for mycological examination. While human and animal specimens were examined by direct microscopy (KOH) and cultured on Dermasel agar, DTM, Derm-Deut and In-Try DM, soil samples were cultured by hair-baiting technique then the growth transferred to the other media. The obtained dermatophytes were identified by both phenotypic including macro morphology, micro morphology as well as culture on differential media (RLA, BCP, MHB and RG) and molecular methods including RFLP, (GACA)4 as well as DNA sequencing.