الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 133 |  |  |
| | | from | 133 | | |
Abstract
The study presented in this thesis include development and application of
some analytical techniques namely fluorimetry and spectrophotometry for
the determination of some active drugs components (e.g. gatifloxacin,
ciprofloxacin) and glucose.
The thesis is divided into 4 chapters:-
Chapter one :-
Includes a general introduction on lanthanides and other methods used for
determination of gatifloxacin, ciprofloxacin and glucose.
Chapter two :-
Describes a highly selective and sensitive spectrofluorimetric method for
the assessment of gatifloxacin in pharmaceutical tablets and serum
sample based on the enhancement of luminescence intensity of the optical
sensor Tb3+ ion . In ammonia buffer solution (pH = 8.0) the gatifloxacin
can remarkably enhance the luminescence intensity of Tb3+ ion in Tb3+-
GTX complex at λex= 340 nm. The produced luminescence intensity of
Tb3+- GTX complex in Acetonitrile is proportional to the concentration of
CFX and can used as optical sensor for its determination. Limit of
detection (LOD)= 1.6 x 10-8 mol L-1.
Chapter three:-
Describes a highly selective and sensitive spectrofluorimetric method for
the assessment of ciprofloxacin in pharmaceutical tablets and serum
sample based on the enhancement of luminescence intensity of the optical
sensor Eu3+ ion. In ammonia buffer solution (pH = 6.0) the ciprofloxacin
can remarkably enhance the luminescence intensity of EU3+ ion in EU3+-
CFX complex at λex = 365 nm . The produced luminescence intensity of
Eu3+- CFX complex in Acetonitrile is proportional to the concentration of
CFX and can used as optical sensor for it’s determination. Limit of
detection (LOD) = 3.3 x 10-9 mol Ll-.
Chapter four:-
A novel spectroflourimetric probe for determining glucose in human serum
samples.is used The optimal experimental conditions of working solvent,
pH and also the concentration of Eu-(CFX) doped in sol gel matrix
fluorophore corresponding to maximum fluorescence intensity were
investigated. Under optimal conditions, the quenched fluorescence
intensity of Eu-(CFX) doped in sol-gel matrix is proportional to the
concentration of glucose in the range 5.0 × 10−9 to 1.0 ×10−6 mol L−1 (r =
0.994). The detection limit (S:N = 3) (LOD) is 2.9 × 10−9 mol L−1. This
method is used for determination of glucose in serum of diabetic patients