الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Livin is an anti-apoptotic protein belonging to the inhibitor of apoptosis (IAP) family, which contains, until the moment, eight members, comprising Livin and survivin. IAP family works by rendering cancer cells insensitive to pro-apoptotic stimuli and this will lead to suppress apoptosis. The main aim of the this study was to evaluate the role of livin- and survivin-targeting siRNA in the down regulation of the corresponding gene expression in three malignant cell lines; Lung (A519), Cervical (HeLa) and Colon (HCT 116), along with one normal monkey kidney cells (Vero). Lipofection-based transfection was carried out to introduce livin-targeting and survivin-targeting small interference RNA (siRNA) into the cancerous cells. Livin and survivin expression was monitored by Real Time-PCR. MTT assay was also employed to assess the cell viability through assessing the mitochondrial reductase enzyme activity. Trypan blue test was done to check the integrity of the cell membranes after being transfected. The data obtained showed that transfecting the three malignant cells with livin- and survivin-specific siRNA have resulted in downregulation of their corresponding gene expression in comparison to cells that were not transfected. Here we concluded that siRNA-mediated gene silencing could be used as a non-conventional, promising therapeutic tool for in cancer treatment in the near future.