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Abstract
ABSTRACT
Ibrahim Shawki Ramadan Abou Soliman: Identifying some Genes Affecting Production Traits of the Maghraby Camel Breed. Unpublished M.Sc. Thesis, Department of Animal Production, Faculty of Agriculture, Ain Shams University, 2014.
Twenty-three Maghraby camels (Camelus dromedaries) were identified and used to detect some genes that affected some growth traits by estimating some productive traits and by using molecular genetics techniques (gene isolation and sequencing). Estimation of live body weights as birth weight, weaning weight, yearling weight and dam weights in the present study revealed that these weights were not high in maghraby camel breed compared with other breeds. Also estimation of daily growth rate for calves from birth to yearling age was less than the other breeds. Genetic analysis of growth hormone gene (GH) in maghraby camel breed showed that sequence of this gene was resulted in 1726 bp by using four specific primers of Sudanese camel breed. Comparison of sequence of GH gene in maghraby breed with the reference of Genbank identified one single nucleotide polymorphism (SNP) in a non coding region (intron 1) in position g.419C>T where there are two alleles (C or T) in this position of SNP. GH sequence also identified 14 position for restriction enzymes. Sequence of Myostatin gene in maghraby breed can also be detected by using a specific primer of Pakistanian camel breed and showed no polymorphism between individuals which mean that this gene was conserved.
Key Words: Camels, Maghraby breed, production traits, growth hormone gene, Myostatin gene, polymorphism, SNP.