الفهرس 
INTRODUCTION AND AIM OF THE WORKOnly 14 pages are availabe for public view
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Abstract
SUMMARY
Aim of the study
The purpose of this study is to clarify the impact of
technique of umbilical cord blood collection on amount and
bacterial contamination of the collected blood.
Methodology
The present study was conducted on a random cohort of 120
deliveries at Maternity Hospital, Ain Shams University and
Ghamra Military Hospital.
UCB samples were collected after placental delivery (exutero).
The samples were divided into 2 groups according to the
method of UCB collection:
• Group 1 60 UCB samples were collected by blood bag
method , 14 samples were discarded due to incomplete
procedures, clotting of the blood or any exclusion criteria,
so 46 samples were included in this group.
• Group 2 60 UCB samples were collected by syringe
method , 6 samples were discarded due to clotting of
blood or any exclusion criteria, so 54samples were
included in this group.
Summary
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Inclusion criteria:
All deliveries will be included as long as consent was taken from
parents for cord blood collection.
Exclusion criteria:
The cord blood will not be collected if there is:
• Maternal history of jaundice.
• Maternal hepatitis or infectious diseases.
• Maternal inherited blood diseases.
The cord blood will be discarded if:
• Any Congenital anomalies.
• Chromosomal disorders.
• Suspected inborn errors of metabolism.
Comprehensive history taking was done for all stressing on:
Pre-existing maternal problems; Fetal problem: and Natal and
Postnatal history.
Detailed clinical neonatal examination :Determination of
the gestational age using Ballard score , APGAR score at 1 & 5
minutes, Determination of birth weight, length and occipito -
frontal circumference and Cord and Placental examination and
weight and Neonatal examination.
Summary
122
Results
This cross sectional study included 100 newborns:48 (48%)
were males and 62 (62%) were females, with GA of
36.290±3.453weeks ranged between , birth weight
2912.33±767.705gm, length 47.740±3.828 cm and OFC was
33.990±2.129cm. The placental weight was 644.14±151.361 gm
and ranged between 284 to 1250, and cord length was
56.20±11.70 cm and ranged between 19 to 133.
The APGAR score at 1 min ranged between 4-10 with
median 7, while at 5 min it ranged between 7-10 with median 9.
Maternal age ranged between 25 to 32 with mean±SD
27.37±1.48 years, weight was 77.91±3.92 Kg and ranged between
70 to 87and parity ranges between 1 to 6 with median 3,mother
Hb% ranged between 11-13.5 % with mean±SD of 12.10±0.77
Twenty seven neonates were delivered by NVD(27%) and
73 with CS (73%) , 28 deliveries with general anesthesia(28%) ,
45 with spinal anesthesia(45%) and 27 with no anesthesia(27%).
Cord blood samples collected with bags were 46 samples
(46%) and with syringe 54 samples (54%).
Regarding maternal factors, 11 (11%) mothers had
hypertension while only 7 (7%) had preeclampsia, 10 (10%) had
gestational DM and all of them did not have fever during that
pregnancy.
Summary
123
The amount of collected blood is positively correlated with
gestational age, neonatal weight, neonatal length, OFC (r=0.434,
p<0.001) and placental weight
There was no correlation detected between maternal age,
weight, Hb or APGAR score with the amount of collected blood.
There was statistically significant higher amount of blood
and lower time of collection in Bag group compared to Syringe
group
The amount of collected blood was positively correlated
with TNC (r=0.699, p=0.003) and negatively correlated to RBCs
count (r= -0.253, p= 0.014) and HCT (r=-0.258, p=0.012) values.
Positive bacterial culture occurred significantly more in the
NVD group (44.44%) compared to the CS group (9.59%) , more
in syringe method (22.22% compared to bag method (15.21%) by
Fisher exact test (p=0.04 & 0.05 , respectively).
Non-statistically significant difference between studied
groups regarding culture results in day one or day seven was
detected.
There was statistically significant lower TNC, TLC,
Lymphocytes, monocyles, esinophils, granulocytes, RDW and
PDW in Bag group compared to Syringe group. Non-significant
difference between both groups regarding the rest of CBC
parameters was detected.
Summary
124
The logistic regression showing that after elimination of all
other factors, there was highly statistically significant association
between higher amount of collected cord blood and GA, mode of
delivery, maternal preeclampsia, TNC and TLC and a statistically
significant association between higher amount of collected cord
blood and maternal weight, placental weight and maternal
hypertension.
The logistic regression showing that after elimination of all
other factors, there was highly statistically significant association
between positive culture of collected cord blood and GA and
mode of delivery and a statistically significant association
between positive culture of collected cord blood and maternal
preeclampsia, TNC and TLC.