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Abstract Proteases are the second most important industri&l enzymes currently after amylases. About 500 tons of these enzymes (based on the pure protein) are produced per year. Proteases are prooduced commercially from both bacteria and fungi. An important distinguishing feature is the optimum pH of the protease. Proteases in the market include alkaline. neutral and acid proteases. The most important producers of the alkaline proteinases are Bacillus strains. Enzymes used in detergents are mainly alkaline proteases from Bacillus strains (Bacillopeptidases). The best known proteases are subtilisin Carlsberg from ~ licheniformis and subtilisin BPN and subtilisin Novo from ~ amyloliguefaciens. These enzymes contain serine at the active site of the molecule and are not inhibited by ethylenediaminetetraacetic acid (EDTA) but are inhibited by diisopropyl fluorophosphate CDFPl. These alkaline proteases excellent from alkaloph:ilic microorganisms characteristics not only in activity but solubility under highly alkaline conditions. |