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Abstract Summary and conclusion 451 The aim of this study was to compare between two new rotary systems GTX and TF through their Shaping properties regarding the Preservation of canal shape before and after preparation, the Amount of extruded debris from the apex and Instrument cyclic fatigue; and their obturating properties regarding their Sealing ability of the obturation Systems of the rotary systems, the Ease of obturator removal for retreatment, the Postoperative pain and The obturators radiodensity. I. Shaping properties: 1.Preservation of path Twenty extracted human mandibular first molar were selected with mesial root curvature above twenty degrees of curvature. Teeth were scanned using Scanora3D machine. Image reconstruction was performed using Ondemand3D software version 1.0.9. The dentine thickness was measured at the mesial and distal sides of the mesiobuccal root canal in each tooth at the levels of 3, 5, and 8 mm from the root apex and the measures were saved for comparison with the postoperative scans. The selected twenty extracted teeth were randomly classified into two equal groups according to the NiTi rotary systems used. In group (1): 10 teeth were prepared using GTX rotary system while in group (2) 10 teeth were prepared using TF rotary system. Summary and conclusion 455 A post instrumentation CT scan were performed to all the teeth under examination and measurement of the mesial and distal aspects of the mesiobuccal root canal were done. The preinstrumentation and post-instrumentation measurements are recorded, tabulated and statically analyzed. Three sections were selected for each tooth at 3, 5 & 8 mm from the root apex. Three way-ANOVA has been used to the effect of Rotary file system, Wall type and level on mean percentage of change in canal path. Tukey’s post-hoc test was used for pair-wise comparison between the means when ANOVA test is significant. One Way- ANOVA was used to study the effect of different level tested and the interactions between all groups on mean percentage of change in canal path within each group. Independent t-test has been used to study the difference between different rotary system and tested wall on mean percentage of change in canal path. The results came as follows: Concerning the effect of the type of the file on preservation of root canal path in the coronal level; TF file system showed significantly higher mean percentage change than GTX on the mesial wall. While an insignificant difference resulted between GTX and TF file system on mean percentage of change in canal path at the distal wall. In the middle level an insignificant difference resulted between GTX and TF file system on mean percentage of change in Summary and conclusion 451 canal path at both the mesial and distal wall. In the apical level, an insignificant difference resulted between GTX and TF file system on mean percentage of change in canal path at both the mesial and distal wall. Concerning the effect of the region (root level) (GTX) System showed an insignificant difference resulted between coronal level middle level and apical level on mean percentage of change in canal path at both the mesial and distal wall. While (TF) System showed an insignificant difference resulted between Coronal Level, Middle level and Apical level on mean percentage of change in canal path at both the mesial and distal wall. 2. Evaluation of the amount of extruded debris from the apical foramen Twenty human freshly extracted single rooted mandibular premolars. The teeth were randomly divided into two groups, group 1 and group 2, according to the type of rotary file used. In group 1: the canals were instrumented with GTX NiTi rotary system, while in group 2: the canals were instrumented by Twisted File NiTi rotary system.. Teeth under investigation were put in the sealing cap of glass test tubes so that the apical 3 mm of the root end were piercing it. The empty test tubes were weighted individually on a 0.01 accuracy electronic scale. The sealing cap with the tooth in it Summary and conclusion 451 was applied to the test tube and prepared according to its group as mentioned previously in the evaluation of preservation of path. The debris was collected, the sealing cap with the tooth inside was removed and the tubes were weighted again. The difference between each tube’s weight pre-operative and post-operative will represent the weight of the debris extruded from the canal. The results showed an insignificant difference between GTX file system and TF File system. 3. Evaluation of the cyclic fatigue resistance Ten new and ten used rotary NiTi files size #30 and 6% tapers were selected from each system for use in this study. Samples were classified into two main groups according to the type of alloy used to manufacture rotary NiTi files.Group1: a total of ten rotary NiTi files (GTX) of identical size (#30) and taper (0.06) which was subdivided into subgroup 1a: representing new files and subgroup 1b: representing old files.Group2: a total of ten rotary NiTi files (TF) of identical size (#30) and taper (0.06) which was subdivided into subgroup 2a: representing new files and subgroup 2b: representing old files. Instruments from each system were tested from cyclic fatigue resistance in a 45 degrees replicated curvature in the apparatus. The handpiece of an endodontic motor was mounted on the apparatus; each instrument was tested in the canal having its same Summary and conclusion 451 dimensions and precisely positioned to the same point in the block to ensure that it will not be displaced, instruments were rotated till fracture. Instruments fracture was visually and audibly detected, and the time to fracture in seconds was recorded by using a digital stopwatch manually operated, started at the moment the motor was turned on, and stopped at fracture detection. All fatigue tests were performed by the same operator. The number of rotations to fracture was then calculated using the simple formula. No. of rotations= x time taken to fracture (in sec.) The fractured fragments were collected and random samples were chosen to be tested under scanning electron microscope. Data was collected, tabulated and statistically analyzed using independent t-test. Results cames as follows: Concerning the effect of file condition, a significant difference resulted between new files and used files on mean cyclic fatigue for GT series X (GTX) system. Also for the twisted files (TF) system, the new files produced the highest significant values for cyclic fatigue compared to used files. Summary and conclusion 451 Concerning the effect of rotary file used a significant difference resulted between new GT Series X (GTX) and new Twisted Files (TF) system on mean cyclic fatigue. Also Used GT Series X (GTX) Files for produced the highest significant values for cyclic fatigue compared to Used Twisted Files (TF) system. Scanning Electron Microscope: Examining the fractured segments of both rotary systems with the SEM under different scales revealed many observations about the behavior of instruments in their failure. New and used GTX rotary files showed crack propagation at the peripheries of the fractured surface and micro-cracks following the grinding marks and some plastic deformation near the fractured surface. Cross sectional studies showed areas of elevations and depressions with rolling of the peripheries suggesting a ductile pattern of fracture. Small black holes in some dimples were noted, those were Nickel Titanium Oxide particles. While regarding TF rotary files, the path of crack propagation showed a tortuous, nonlinear path that was not related to the surface texture of the TF instruments. Summary and conclusion 411 II. Obturation properties of systems under investigation 1. Evaluation of the sealing ability of the obturation systems Teeth investigated in extruded debris were used in this study. Twenty root samples were divided into two equal groups (n=10) according to the system used for preparation. In group (I) the teeth were cleaned and shaped using GTX rotary NiTi instruments, while in group (II) 10 teeth were cleaned and shaped using TF rotary NiTi instruments. This was in addition to four teeth; two teeth were used as negative controls and other two teeth were used as positive control samples for the study. The canals were dried using paper points size #30; roots were obturated using cold lateral condensation technique. Group (I): A standardized size #30, 0.4 taper GTX gutta-percha cone was dipped in and out of the canal to introduce the sealer along the whole length of the canal. Group (II): A standardized size 30, 0.4 taper resilone cone was dipped in and out of the canal to introduce the sealer along the whole length of the canal. Two way-ANOVA have been used to evaluate the effect of the rotary file system and time on mean microleakage. Tukey’s posthoc test was used for pair-wise comparison between the means when ANOVA test is significant. One Way-ANOVA was used to study the effect of time on mean microleakage. Independent t-test has been used to study the difference between different rotary systems on mean microleakage. Results showed the following: Summary and conclusion 414 Concerning the effect of different rotary system used on mean sealing ability regardless of time variable Twisted files showed significantly higher mean microleakage than GTX. Concerning Effect of time on mean sealing ability regardless of different rotary system used variable non-statistically significant decrease in microleakage was observed after one week. Three weeks period showed statistically significantly lower mean microleakage than 24 hours period. One week period didn’t show significant difference from 24 hours or 3 weeks period. Concerning Effect of rotary file system used on mean sealing ability for each time. One week produced an insignificant difference after storage followed by an insignificant decrease after 3 months of storage on mean microleakage for (GTX) file system. Also On week produced an insignificant difference After storage followed by an insignificant decrease after 3 months of storage on mean Microleakage for Twisted Files (TF) file system. While the effect of rotary file used an insignificant difference resulted between GTX and TF file system on mean Microleakage at 24 hours. Also an insignificant difference resulted between GTX and TF file system on mean microleakage at one week. On the same way, an insignificant difference resulted between GTX and TF file system on mean microleakage at 3 weeks. Summary and conclusion 411 2. Evaluation of the ease of obturator removal The twenty extracted teeth used in the sealing ability study were radiographed in a bucco-lingual direction. Roots were standardized using a diamond disc operated at low speed to be 16 mm in length. Filling material removal was judged complete when the working length was obtained, and no more gutta-percha or resilon could be removed with the instruments. The preparation time in seconds to complete the retreatment procedures was recorded, not including time for change of instruments and irrigation. The roots were then grooved longitudinally, and then split by using a chisel. Ten halves of each group were gold plated and examined under Scanning Electron Microscope. The other parts were examined under stereoscope to calculate the remaining debris. Independent t-test has been used to study the different between rotary file used on mean extruded debris and time of treatment in Ease of treatment. Two way-ANOVA have been used to the effect of Rotary file system and tested level on mean percentage of remaining debris. Tukey’s post-hoc test was used for pair-wise comparison between the means when ANOVA test is significant. One Way-ANOVA was used to study the effect of level tested on mean percentage of remaining debris. Independent t-test have been used to study the difference between different rotary system on mean percentage of remaining debris. Summary and conclusion 411 Concerning the Effect of rotary File system used on mean time an insignificant difference resulted between GTX file system and TF File system. While the effect of rotary file system and tested level on mean remaining debris. Two way-ANOVA shows that Rotary file system and tested level produced an insignificant effect on mean Remaining debris (%). Concerning effect of different rotary system used on mean remaining debris (%) regardless of time variable an insignificant difference resulted between GTX and TF file system on mean Remaining debris (%). Concerning Effect of tested level on mean Remaining debris (%) regardless of different rotary system used variable: An insignificant difference resulted between coronal level, middle level and apical level. Concerning the effect of rotary file system used on mean remaining debris (%) for each level tested: An insignificant difference resulted between coronal level Middle level and apical level for GT Series X® (GTX) file system. Also an insignificant difference resulted between coronal level, Middle level and apical level for Twisted Files (TF) file system. Summary and conclusion 411 While regarding the effect of rotary file used an insignificant difference resulted between GTX and TF file system on mean Remaining debris (%) for coronal level. An insignificant difference resulted between GTX and TF file system on mean Remaining debris (%) for Middle level. An insignificant difference resulted between GTX and TF file system on mean Remaining debris (%) for Apical level. 3. Postoperative pain Twenty patients with age varying from 18 to 42 years old suffering from mandibular first molar teeth acute pulpitis without apical periodontitis and sound periradicular bone radiographically. They were divided randomly into two groups; Group A, the canals were obturated with gutta-percha cones and AH plus sealer using the lateral condensation technique. Group B, the canals were obturated by resilone points and AH plus sealer. No systemic medication was prescribed; a chart was given to each patient to record the postoperative pain after 6, 12, 24 and 48 hours. A non-parametric one-way ANOVA (Kruskal–Wallis) test followed by paired group comparisons using Mann–Whitney U tests at a 5% significance level were used to analyses pain intensity scores statistically for the effect of time. Mann-Whitney U test have been used to test the statistical difference of the rotary systems used on pain intensity scores. GTX group was a statistically significant Summary and conclusion 415 lower in pain scores through all periods. While for TF group, there was non-statistically significant decrease in pain scores from 6 hours to 12 hours. Then a statistically significant decrease was observed from 12 hours to 24 hours as well as from 24 hours to 48 hours. Concerning the rotary file system There was no statistically significant difference between the two systems through all periods. 4. Radiodensity After finishing the root canal treatment a periapical radiograph was taken and comparison the radiodensity by mean of Gray Scale Value. Independent t-test has been used to study the different between rotary file used on mean radiodensity. Data were presented as mean and standard deviation (SD) values. Data were explored for normality using Kolmogorov-Smirnov and Shapiro-Wilk tests. Percentage change in canal path as well as pain scores showed non-parametric distribution while all other variables showed normal (parametric) distribution. For parametric data; Student’s t-test was used to compare between the two systems as well as to compare between cyclic fatigue of new and used files. Two-way Analysis of Variance (ANOVA) was used to study the effect of file system and storage time on sealing ability. Tukey’s post-hoc test was used for pair-wise comparisons when ANOVA test is significant. Summary and conclusion 411 For non-parametric data, Mann-Whitney U test was used to compare between the two systems. This test is the non-parametric alternative to Student’s t-test. Friedman’s test was used to compare between root levels or different time periods within the same group. Wilcoxon signed-rank test was used for pair-wise comparisons when Friedman’s test is significant. An insignificant difference resulted between GTX file system and TF File system on mean Radiodensity (Gray Scale Value). Under the experimental condition of this study, the following conclusions could be obtained: Instruments produced by M-wire and R-phase proved to have an almost equal performance regarding shaping ability. Instruments manufactured using M-wire technology proved to have a superior cyclic fatigue resistance than instruments manufactured using R-phase technology. Gutta percha is still the gold standard for evaluating new root canal core filling materials. Whatever is the instruments used in canal shaping or the obturation material used, removal of the canal obturation needs further interference. Postoperative pain in both systems was similar. Regarding the radiopacity, resilone was found to be more radiopaque than gutta percha, but both still meet the ADA requirements for ideal obturating materials. |