الفهرس 
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Abstract
The aim of this in-vivo study was to prepare specified standardized extracts from certain natural plants: (Green tea leaves, Cinnamon bark and Olive leaves) and to compare them with calcium hydroxide intra-canal medicament regarding: The biocompatibility of these drugs and their antimicrobial activity. Materials and methods: In the first part of the study, the selected drugs were air dried, cut into pieces and ground into powder form. The extracts were prepared using different solvents, fractionated using solvents of different polarity .Then, screened against different bacteria and the most potent extracts were chosen for the study. In the second part of the study, the subcutaneous implantation technique was carried out on forty male albino Sprague-Dawley rats. The rats were classified into four groups, ten rats each, according to the tested material and the experimental period: Group I: Green tea extract, cinnamon bark extract and negative control. (14 days observation period), Group II: Olive leaves extract, Meta paste and negative control (14 days observation period), Group III: same as Group I (30 days observation period) and Group IV: same as Group II (30 days observation period). In the third part of the study, thirty two patients free of systemic diseases were selected. All the selected upper anterior teeth were diagnosed as having pulpal necrosis. The first microbiologic sample was collected after opening the access cavity by placing four sterile paper points in the canal for 1 minute each. The paper points were then placed in eppindoeffes containing reduced transport fluid and sent for microbiological evaluation. Chemomechanical preparation was performed using the hybrid technique with copious irrigation. After drying the root canals with sterile paper points, a post instrumentation sample was obtained as the previous manner. The teeth were then randomly divided into four groups according to the type of intracanal medication: Group I: Green tea leaves extract, Group II: Cinnamon bark extract, Group III: Olive leaves extract and Group IV: Meta paste. After 7 days the canals were reopened, irrigated with saline and a final root canal sample was obtained as previously mentioned. Screening and counting of Candida albicans and Enterococcus faecalis was performed. Results: Results showed that green tea extract was the most biocompatible material and that cinnamon bark extract gave the highest percentage reduction of the bacterial and candidal counts followed by green tea extract then meta paste and the least was reported to olive leaf extract.