الفهرس | Only 14 pages are availabe for public view |
Abstract The present study was concerned to examine the gene expression of IL-1B, IL-6, TNF-a and TLR-4 due to lIP infection of MD RSA in mice as a pro inflammatory cytokines. The current study included two groups of mice. The first group (n= 10) was treated as a control group, injected with normal saline. The second group was divided into six replicates (n= 10 for each replicate). The replicates were intraperitoneal injected with a fresh culture of (MDRSA). Mice were sacrificed after 6, 12, 24, 48 and 72 hr and 7 days from infection with bacteria. Then liver and spleen tissues were collected. To fu1fill these aims, specific objectives were achieved. These objectives included: studying the induced inflammation related genes by sequencing the RT-PCR product and using BLAST tools in Gene bank website and other tools on other websites (expasy tools) for prediction and characterization of studied genes. And studying histopathological changes in liver and spleen tissues. Results of this work showed that: (l) There were no expression for IL-l P expression pattern in control non infected mice liver tissue and that bacterial infection led to . a marked increase in IL-1 P gene expression in liver tissue detected after 12, 24 and 48 and 72 hr but no expression at 6 hr and 7 days post infection. (2) There were no expression for IL-6 genes in normal uninfected mice liver while IL-6 expression markedly increased in liver tissue after the bacterial infection at 12, 24 and 48 hr but no expression at 6 and 72 hr and 7 days. (3) There were no expression for TNF-a genes in normal uninfected mice liver while TNF-a expression markedly increased in liver tissue |