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العنوان
Studies on some prointlammatory cytokines gene expression after infection with Escherichia coli 0157:H7 /
المؤلف
AbdEl-Ghfaar, Diana Atef Saed.
هيئة الاعداد
باحث / Diana Atef Saed AbdEl-Ghfaar
مشرف / Ahmed M. Ammar
مشرف / Gamal A. El-Mowalid
مشرف / Mohamed A. Kutkat
الموضوع
Veterinary Immunology. Infections. Cytokines- genetics.
تاريخ النشر
2014.
عدد الصفحات
91 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - Bacteriology, Mycology and Immunology
الفهرس
Only 14 pages are availabe for public view

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Abstract

The present study was conducted to examine the E. coli 0157:H7 in
mice and induction of Cytokines on the transcriptional activity of the
inflammation. The current study included two groups of mice. One
group was treated as control group and injected by physiological
saline while second group divided into six groups were injected by E.
coli 0157:H7. Each group was composed of ten mice (n=10). All
mice in the six replicates groups developed Haemorrhagic colitis.
This was proved, after sacrificing the animals on different intervals of
time (after 6, 12, 24, 48 and 72 hr and 7 days), through a series of
molecular and histological investigations. However, the animals
infected with bacteria showed some signs of illness that can be
noticed exteriorly.
To fulfill this aim, specific objectives were achieved. These
objectives included: prediction of protein sequence for the studied
induced inflammation related genes by sequencing the RT-PCR
product and using BLAST tools in Gene bank website and other tools
on other websites (expasy tools).