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Abstract A total of one hundred and twenty random samples of different meat products (Beef burger, Luncheon, Canned corned beef, Sausage,bPastirma and Kofta) (20 of each) were collected from different supermarkets in Menoufia governorate. The samples were subjected to chemical determination of NaCl (%), nitrite content (ppm), phosphate (%), ascorbate level (ppm) and the presence of monosodium glutamate in the examined meat products. The mean value of NaCl % in Beef burger, Luncheon, Canned corned beef, Sausage, Pastirma and Kofta was 2.54± 0.14, 3.24± 0.09, 3.66± 0.13, 2.93± 0.17, 8.48± 0.12 and 2.92± 0.16 respectively. With minimum value of 1.5, 2.6, 1.9, 6.7 and 4.3(%) and maximum value of 4, 4, 4.7, 4.4, 9.4 and 1.9(%), respectively for the mentioned meat products. the percentage of samples exceeded the permissible limit was 70 % for beef burger, 65% for luncheon, 85% for canned corned beef, 35% for sausage, 75% for pastirma and 40% for kofta. This according to Egyptian Standard Specification (EOS) (2005). The mean values of nitrite levels (ppm) in the examined meat products as follows 94.04± 5.20 for beef burger, 98.65± 3.41 for luncheon,42.30± 2.47 for canned corned beef, 127.15± 6.24 for sausage, 48.69± 4.68 for pastirma and 79.56± 6.25 for kofta. The minimum and maximum values of nitrite in meat products (ppm) were showed as 58- 131, 68-127, 25-62, 72-172, 28-94 and 37-123 for beef burger, luncheon, canned corned beef, sausage, pastirma and kofta, respectively. 30% of beef burger samples exceed the permissible limit, 45% of luncheon samples exceed this limit as well as 25% of canned corned beef, 85% of sausage, 25% of pastirma and 15% of kofta. The levels of phosphate (%) in the examined meat products that the mean value of phosphate was 0.43± 0.02 for beef burger, 0.41± 0.02 for luncheon, 0.43± 0.019 for sausage and 0.38± 0.029 for kofta. Phosphate failed to be detected in the examined canned corned beef and pastrami samples while failed to be detected in 10%, 60%, 20% and 20% in the examined beef burger, luncheon, sausage and kofta samples, respectively. The detectable samples above the permissible limit were 75%, 35%, 75% and 55% for beef burger, luncheon, sausage and kofta samples, respectively. The mean value of ascorbate in the examined meat products was 487.82± 22.84, 435.3± 36.91, 503± 2.49, 417.67± 20.08, 398.17±7.94 and 461.29± 15.78 (ppm) for beef burger, luncheon, canned corned beef, sausage, pastirma and kofta, respectively. The detectable samples above the permissible limit recommended by ES (2005) were 30% of the examined beef burger samples, 45% of the examined luncheon samples, 10% of the examined canned corned beef samples, 10% of the examined sausage samples and 15% of the examined kofta samples The incidence of monosodium glutamate in the examined meat products revealed that 50 %, 45%, 10% and 5% of the examined beef burger, luncheon, sausage and kofta showed positive samples for monosodium glutamate. Experimental work: The effect of nitrite (172 ppm), ascorbate (582 ppm), phosphate (0.62%), (nitrite 172ppm + ascorbate 582 ppm) and (nitrite 172 ppm + ascorbate582 ppm + phosphate 0.62%) on liver and kidney functions in comparison to control group were discussed after 2 weeks of experimental work. They all showed higher mean values of GOT, urea, uric acid, serum creatinine and body weight in comparison with control group while lower mean values of GPT and alkaline phosphatase. Histopathological examination : The histopathological examination showed mild lesions in liver, kidney and heart while no lesions were noticed in spleen of the examined rats treated with 172 ppm nitrite, mild changes in liver, kidneys, heart and spleen in the examined rats treated with 582 ppm ascorbate, mild changes in liver, kidneys, heart and spleen of the examined rats treated with 0.62% phosphate, mild to moderate lesions in liver and kidneys, and mild changes in heart and spleen of the examined rats treated with 172 ppm nitrite + 582 ppm ascorbate while moderate lesions in liver, kidneys, heart and spleen of the examined rats treated with 172 ppm nitrite + 582 ppm ascorbate + 0.62% phosphate . from this study we can summarized that chemical preservatives explained in this experimental work with the referred doses lead to hazardous effects on liver, kidneys, heart and sometimes spleen. |