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Abstract A. hydrophila is the most common bacteria in fresh water habitats throughout the world, which it causes motile Aeromonas Septicemia (MAS), and both antibiotics and probiotics have the ability to resist bacterial diseases, and it found that they are effective against MAS beside that the use of antibiotics and probiotics improve fish health, and growth rate. This study was carried out on 362 Clarias gariepinus obtained alive from Muwies canal at Zagazig city. A total number of 50 Nile catfish of different body weight which were showed signs of Hemorrhagic Septicemia (pale gills, bloated appearance, and skin ulcerations) used for A. hydrophilas isolation, the rest 312 Nile catfish were divided in to two equal groups to study prophylactic and treatment effect of antibiotics and probiotics. Each group which consists of 156 Nile catfish allocated into thirteen groups in two replicates according to their body weights. G 1 was regularly fed with basal diet; G2, 3, and 4 were regularly fed with basal diet supplemented with oxytetracyline at concentration of 0.3, 0.6, and 1.2g/kg diet respectively; G5, 6, and 7 were regularly fed with basal diet supplemented with florofenicol at concentration of 5, 10, and 15ml/kg diet respectively; G8, 9, and 10 were regularly fed with basal diet supplemented with biogen at concentration of 0.5, 1, and 2g/kg diet respectively; and G11, 12, and l3 were regularly fed with basal diet supplemented with yeast at concentration of 0.5, 1, and 2g/kg diet respectively. One divide used to prophylactic study, in which feeding rate was 5% from total weight and four times daily, fish of each group were weighted every four weeks during sixteen weeks for |