الفهرس 
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Abstract
Nigella sativa (black cumin) used as a natural remedy for a number of illnesses that include asthma, cough, hypertension, bronchitis, eczema, fever, inflammations, and other diseases. Crude extracts have shown effectiveness against multi antibiotic resistance bacterial isolates. The current research aim to detect the antibacterial activity of Nigella sativa oil extract. To achieve this aim .
The following was done and obtained results were as follow:
1) Isolation and identification of 28 isolates obtained from 67 samples from chicken and ostrich. The serological identification of these isolates was done and the results revealed that 11 different serotypes were identified among E.coli isolates and the most predominant one was serotype O103 with 32.1% followed by O26 and O78 with 14.2% and 10.7% respectively while O91, O119, O126 and O158 have the same percentage which was recorded as 7.1%. In contrast O127 has the lowest percentage which was recorded as 4%.
2) Examination of 28 isloates of E.coli against 13 antimicrobial agents and N.sativa oil extract revealed that of E.coli isolated from chicken showed that all isolates were susceptible to colistin and gentamycin followed by ciprofloxacin (86.9%) then sulfamethoxazole/ trimethoprim (78.2%) which considered as most effective antimicrobials and drugs of choice. Erythromycin was (0%) and forE.coli isolates from ostrich, all isolates were susceptible to colistin, gentamicin, ciprofloxacin, danofloxacin, nalidixic acid, Chloramphenicol and ampicillin followed by SXT 80%., while all isolates from chickens and ostrich were resistant to Nigella sativa oil extract.
3) Concerning susceptibility testing of the23 S .aureus isolates to different antimicrobial agents all isolates were susceptible to gentamicin (91.4%) followed by rifampicin (82.6%), sulfamethoxazole trimethoprim, amoxicillin clavulantic acid, erythromycin were effective. also S.aureus isolates were susceptible to N.sativa oil extract (91.4%).