الفهرس 
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Abstract
Poly vinyl chloride is a poison plastic which consider the most environmentally harmful plastic, it well established as animal and human carcinogen. The present study aimed to early investigation of carcinogenic risk
of poly vinyl chloride in order to improve cancer therapy through
studying the toxic effect of PVC on growth performance, hematological
parameters, biochemical changes, alfa-fetoprotein on serum as tumor
marker, m-RNA expression of cytochrome P450 2E1 (CYP2E1) and
tumor suppressor gene (TP53) as early cancer biomarker and
histopathological changes of liver.
Sixty male albino rats were used in this study. These rats were
divided into 3 groups each one contains 20 rats as the following:
Group I was given corn oil only (kept as positive control).
Group II was given PVC at dose 1/20 LD50 (25mg/100gm/bw).
Group III was given PVC at dose 1/10 LD50 (50mg/100gm/bw).
Poly vinyl chloride dose was based on the median lethal dose
(LD50) which reported to be 5000 mg/kg for rats, dissolved in corn oil as a
vehicle. All rats dosed one dose/3 times /week (day after day), orally by
using stomach tube for 2 months. Half of rats in each group were scarified
after first month (4th week) and the other half after second month (8th
week) of the experiment.
The experiment were continued to two months, during which all
groups of rats were observed daily, weighed weekly and clinical signs
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were recorded all over the experimental period. Mean body weight was
calculated by weighting rats every week and given mean body weight
every month.
Collection of blood samples were performed at 4th and 8th weeks of
the experiment. Blood samples were divided into: blood for hematological
studies collected in clean blood collecting tubes with EDTA as an
anticoagulant and serum stored at -20◦C for biochemical analysis.
Tissue Specimens from liver were taken at 4th and 8th weeks and
preserved at (-80) for molecular study. Histopathological studies were
done on liver.
Hematological examination include total erythrocytic count,
hemoglobin concentration, packed cell volume (PCV %), MCV, MCHC,
total leukocytic cell counts and differential leucocytic count. While serum
biochemical parameters include total protein, albumin, globulin, alkaline
phosphatase (ALP), alanine transaminases (ALT), aspartate
transaminases (AST) and alfa-feto protein (AFP).
Tissue Specimens from liver were taken for molecular study of
mRNA expression of CYP2E1, TP35 and CYC genes by semiquantitative Reverse Transcription-polymerase chain reaction (RT-PCR).Specimens from liver were collected immediately after scarification and kept in formalin 20% for histopathological examination.