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Abstract Analytical study of some drugs used in psychiatric disorders with antidepressant effect of forensic medicine interest Lately, psychiatric drugs have found wide use among large sectors of young as well as old patients. Accordingly, it is deemed necessary to propose suitable methods to assay these drugs in their pharmaceutical preparations and in biological fluids. The thesis compromises three main parts: Part I This part contains a short account about psychiatric disorders and different categories of anti-psychotics. Also the pharmacological actions and side effects of each category are discussed. A representative example of two drugs (Aripiprazole (ARP) and Quetiapine (QTI)) under study is given with a highlight on their chemical structures, formulae, chemical names, physical properties and pharmacological effects. Furthermore, it includes literature reviews for the reported methods for the selected drugs in pharmaceutical dosage forms as well as biological fluids. Part II This part deals with different analytical techniques for the determination of ARP in tablet dosage forms and human plasma. It compromises two chapters: Chapter 1: In this chapter, two spectrophotometric methods have been described for the determination of ARP in tablets. The first method was based on measurement of the absolute values of 1D amplitudes at 225nm and of peak to peak at 245 and 268nm. 1D225 and1D245-268 in methanol gave linear calibration curves at concentration ranges of 0.3-1 µg/ml for 1D225 and 2-6µg/ml for 1D245-268. The second method was based on the formation of an ion pair complex between the drug and Alizarin Yellow G and the absorbance of the highly yellow colored product was measured and quantified spectrophotometricaly at 490nm without any extraction step. This complex is formed through the interaction of the protonated form of the basic tertiary amino group of aripiprazole and the carboxylate anion of AYG in an acidic medium of phosphate buffer pH 4.2. Factors affecting color development were studied and optimized for the reaction. |