الفهرس 
English coverOnly 14 pages are availabe for public view
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Abstract
Equine laminitis is a multifactorial connective tissue disorder with major implications for the welfare of horses. Hormonal and alimetary disturbances implicated in such condition. Aiming to monitor the biochemical and molecular changes that occurring during different stages of laminitis, the current study was carried out on 40 male horses attended to the surgery clinic at Faculty of Veterinary medicine, Cairo university. Animals were categorized based on clinical examination into clinically healthy and laminitic (early and late stage of laminitis). Levels of blood glucose, insulin, malonaldialdehyde (MDA), protein carbonyl content (PCC) and DNA fragmentation, activities of pro-matrix metalloproteinase 2 and 9 (pro-MMP2 and 9)and ISSR-PCR using dinucleotide and trinucleotide primers were carried out. Our result indicated a significant increase in the levels of glucose and insulin in laminitic horses with increase in oxidative stress indices (MDA, PCC and DNA fragmentation). Activity of MMP-2 increased in both early and late stage of laminitis, while MMP-9 activity increased only in late stage laminitis. ISSR-PCR using dinucleotide primer ” (GA) 9 C” produced 77 bands from which 18 bands are polymorphic (23.4%) and 59 (76.6%) bands are monomrphic, while ISSR-PCR using trinucleotide “(GAG) 6 C” primer produced 48 bands from which 4 bands are polymorphic (8.3%) and 44 bands (91.7%) are monomrphic.