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Abstract Avian influenza (AI) was among the most emerging zoonoses that threatened humans and poultry worldwide. The aim of current study was to find out protective measures for humans against AI. A total of 480 poultry backyards and 102 human contacts were examined in different seasons for the presence of AI H5N1 in Gharbia governorate using Real time PCR. The overall positivity in backyard poultry Gharbia governorate was 8.7% and the higher risk was in winter. The overall positivity in human contacts was 3.9%. The higher risk of zoonotic transmission of AI H5N1 infection was increased in winter season. Hygienic ventilation, hygienic space and/or raising of poultry in separate species were considered as important factors for the control of AI H5N1 infection in backyard poultry. Absence of access between the backyard poultry and living area of humans, absence of droppings in humans` living area and prevention of direct contact between humans and poultry without applying of protective measures were considered as protective methods for humans against AI and control the disease. |