الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Studies on Gallibacterium anatis infection in chickens Gallibacterium anatis can be regard as a secondary pathogen in chickens but there is at least certain strains seem capable of promoting severe morbidity and even mortality in the immunosuppressed host. Most reports of Gallibacterium species describe the predilection seat for the reproductive tract resulting in salpingitis, oophoritis, peritonitis and sepsis This thesis aimed to investigate the rate of natural infection of G. anatis in chicken flocks, by conventional and modern techniques The study was accomplished by testing 38 chicken flocks (26 layer, 3 broiler breeder and 9 broiler flocks) with no history of vaccination against G. anatis during 2010-2012 Samples collected from diseased and freshly dead birds in these flocks, with cumulative morbidity rate during the week of sampling ranged between 1-15%, while mortality rate ranged between 0.06 - 4.9%. Samples were 89 tracheal swabs, 86 cloacal swabs , 62 oviductal swabs and 61 ovarian samples, respectively The results revealed the following: a) Seven samples from 5 layer flocks (No. 10, 31, 34, 35 and 37) revealed positive results with Gallibacterium infection (5/38) (13.1%). Catalase and oxidase tests were positive while urease and indol test were negative b) Based on PCR and sequencing, some of the isolated strains were identical to G. anatis strain UMN 179 which cause peritonitis and salpengitis in layers causing also DROP in egg production. c) Chloramphenicol, norfloxacin, ciprofloxacin, erythromycin, amoxicillin, ampicillin, cefardine, and gentamycin were the most effective antibiotics against G. anatis, while oxytetracycline, doxycycline, streptomycin, Sulphamethoxazol + trimethoprim, lincomycin and spectinomycin showed complete resistance. d) Statement of more epidemiological survey is badly need to clarify the rate of G. anatis in the problems of egg production in commercial layers as well as the broiler breeder flocks because this is the first record on G. anatis infection in chickens in Egypt.