الفهرس 
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Abstract
In this study 265 examined milk samples were collected from ( 120 from cows milk , 120 from buffaloes milk , 25 goats milk ). The result of CMT refer that there are ( 110 clinically mastitic cows milk samples , 10 subclinically mastitic cows milk samples ,97 clinically mastitic buffloes milk samples ,23 subclinically mastitic buffloes milk samples and 25 subclinically mastitic goats milk samples ).In the present study the prevalence of klebsiella spp isolated from buffaloe ,cow and goat milk samples revealed the percentage of K.pneumoniae was 55.6% and 44.4% from clinically mastitic cow and clinically mastitic buffaloe , respectively although it give zero percentage from clinically mastitic goat. The K.pneumoniae. were distributed as 5 strains from cows,4 strains from buffaloes.The results of virulence study revealed that all 9 Klebsiella isolates were recovered from clinically mastitic cow and buffaloe showed Congo red positive with different degree in red colour,5(55.6%) isolates gave CR++ (deep red) and 4 isolates of which (44.4%) gave CR+ (pale orange) and Klebsiella species on Vero cell induced severe vaculation and detachment. The mortality rates of inoculated mic with the isolated K.pneumoniae within 2hours was 100% within 2hours and the total of 9 K.pneumoniae isolates which were isolated from clinically mastitic cow and buffaloe milk samples were tested for the ability to produce enterotoxin using infant mice assay as enterotoxogenic strains cause accumulation of fluid in the intestinal tract of infected infant mice. The result of the antibiogram profile for 9 K.pneumoniae isolates were susceptible to carbenicillin, cephotaxime, flumequine, gentamicin and kanamicin inan incidence of 100% .Most strains were susceptible to neomycin (86.96 %) and nitrofurantoin (91.30 %). On the other hand, most Klebsiella species were resistant to ampicillin ,chloramphennicol,colistin sulphate,erythromycin,penicillin G and streptomycin,On the other hand, most Klebsiella species were resistant to ampicillin ,chloramphennicol,colistin sulphate,erythromycin,penicillin G and streptomycin. The isolates were screened by using PCR for confirmation by using the primer specific for 16s rRNA ,show the positive amplification of the 130bp fragment of primer specific for the 16s rRNA gene in all of the examined klebsiella pneumoniae (n=9; 100%).
For virulotyping analysis, the isolates were screened for the presence of seven virulence genes (rmpA, k2, k1, uge, aerobactin, kfu and magA genes). The results of the m-PCR revealed positive amplification of products 16s rRNA, rmpA , k2, k1genes at 130 bp16s rRNA of the tested klebsiella pneumonia. 536 bp of the rmpA was found in 7 of the tested klebsiella pneumonia , 641bp of the k2 genes was found in 5 of the tested klebsiella pneumonia ,while 1283 bp of k1 was found in6 of the tested klebsiella pneumonia although the results of the m-PCR positive amplification of products uge, kfu, magA genes at 534, 797 and 1280 bp in 7 of the tested klebsiella pneumoniae , while positive amplification of products of aerobactin gene at 556 bp in 6 of the tested klebsiella pneumoniae.