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Abstract The current work was conducted in El-Zahra Governmental Stud, belonging to the Egyptian Agriculture Organization (E.A.0). Sixteen matyre healthy Arabian stallions were selected for this study. The age of stallions ranged from 4-19 years at the beginning of this work (from December, 1992 to November, 1993 ). To study the effect of age animals categorized into 4 age groups each including 4 stallions. Group I : 4 - 7 years Group II : 8 - 11 years Group III : 12 - 15 years Group IV : 16 - 19 years I-Semen characteristics : To study the effect of seasons on semen characteristics one ejaculate was collected once monthly from each stallion throughout a period of one year, data were grouped by seasons according to the conventional classification as follows : 1-winter (December - February ) 2-Spring (March - May ) 3-Summer( June - August ) 4-Autumn (September-November) Semen collection : semen was collected from stallions using an artificial vagina. The gelatinous material (gel) at the end of ejaculate was separated. Evaluation of gel- free semen was performed. Major sperm abnormalities : Amarked increase in the % of defective spermatazoa was recorded during November. (18.56%). The lower level was observed during October (7.51 %). -Seasons had no any significcance on the percentage of major abnorpmalities. -Semen obtained from young stallion GI contain the highest % of defective sperm. (15.04%) which decline significantly with advancement of age to reach 10.52% in GIII. Minor sperm abnormalities : - The difference between months were non-significant. - The effect of season was non-significant. - Semen obtained from young stallions GI had significant higher % than older stallions of Gil , III and IV. - Proximal protoplasmic droplets : -Monthly differences were statistically non. significant. -The effect of season was non-significant. -The percentage of spermatozoa with proximal dropletes was significant higher in G IV than in other 3 age groups. |