الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 168 |  |  |
| | | from | 168 | | |
Abstract
Two hundred random samples , 50 each of liver ,kidney ,heart and spleen were collected from slaughtered cattle at Benha ,Toukh and El Tall - El Kepeer abattoirs . Each sample was weighed about (50gm) , wrapped separately in aluminum sheets and transferred to laboratory and kept at —20C° to be examined for determination of different organochlorine pesticides ( DDT , DDE , lindane and dieldrin 1 and tetracycline antibiotics residues .
DDT was determined in slaughtered cattle at of 22% , 14% , 6% in liver , kidney and spleen respectively , with a mean
value of 2.11 , 2. 23 and 1.92 ppm in liver , kidney and spleen
samples respectively .One liver sample was exceed the MRLs of DDT.
DDE was determined at 22% , 14% and 6% in liver , kidney and spleen samples respectively , with a mean value of 1.40 , 1.79 and 1.48 ppm in liver , kidney and spleen samples respectively .
Dieldrin had determined in 8 % of liver and 4% kidney samples respectively , with mean value of 0.22 and 0.04 ppm in
liver and kidney samples respectively .Two liver samples were exceeded the MRLs of dieldrin
Lindane ( alpha HCH) had determined in 6% and 4% of liver and kidney samples respectively , with a mean value of 0.37 and 0.22 in liver and kidney samples respectively.
Lindane ( Beta HCH) had determined in 6% liver and 4% kidney samples respectively , with a mean value of 0.35 and 0.27 ppm in liver and kidney samples respectively .
DDT, DDE , Lindane ( alpha HCH) and Lindane (beta HCH) failed to be detected in heart samples however, Lindane (alpha HCH and Beta HCH) failed to be detected in spleen tissues too .
Regarding the effect of cooking by boiling or by frying on the positive samples , the obtained data indicated that there was not any traces of the pesticides tested .
However freezing of the positive samples for up to two months at — 20C° has no significant effect on the pesticide residue level.
The antibiotics were considered as substances help in protection from the diseases ,as well as the treatment of different diseases . In addition , it was used as growth promoters .
The improper use of tetracycline as a broad spectrum antibiotic in animal production , resulted in antibiotic residues in the animal tissues and this hazard affects the human health .
The present study performs to evaluate such residues in the edible offal of animal carcasses as well as the influence of different heat treatments on tetracycline residues .
Tetracycline residues were determined in 26% and 16% of liver and kidney samples respectively , with a mean value of 480.39 and 488.75 ppm in liver and kidney samples respectively
The results indicated that , tetracycline antibiotic failed to be detected in the heart and spleen tissues due to their over circulating blood wash.
Boiling of the positive samples for 30 minutes , roasting at 150 C° for 30 minutes and frying for 15 minutes caused complete residual degradation in the liver and kidney samples.
The positive samples were kept in deep freezer at —20 C° for five months and re-examined monthly for the presence of tetracycline residues , the freezing storage resulted in degradation of tetracycline after four months storage .
The results of detection of tetracycline antibiotic residual levels in collected samples from Benha , Tokhe and El-Tall El-kepeer abattoirs , indicated that detection of drug residues in 13 liver samples and 8 kidney samples. Tetracycline failed to be detected in spleen and heart samples
The recommended measures for slaughtering animals administrated chlorinated pesticides and tetracycline antibiotic with different routes were discussed.