الفهرس 
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Abstract
The present study is carried out on 7 apparently healthy male dogs, aging 2-3 years and weighing 13-18 kgs, on which 35 surgically induced wounds (experimental wounds) were created.
Four drugs were used in this investigation; Propolis, MEBO, Panthenol and Honey/cod liver oil mixture . Propolis was used in form of propolis powder. Honey/cod liver oil mixture was used in form of suspension (50 % of each). Meanwhile, MEBO and Panthenol were used in form of ointment.
35 surgically induced wounds were conducted in the back region of the selected dogs after their physical controlled in sternal recumbent position sedation by 2 % Xylazine HCl injected intramuscular in a dose rate of 1 mg/ kg body weight. 2 % xylocaine HCl was injected in the site of skin incision. After routine aseptic preparation of the wound area in each dog, 5 neighboring rectangular full-thickness skin wounds measuring 3 cm length and 2 cm width for each were conducted. Hemorrhage was controlled by pressure for 2 minutes. The wounds were bandaged with adhesive tape to protect them from contamination and flies and kept for 24 hours without treatment. Immediately before treatment application, each wound was washed with 10% povidone iodine and irrigated with distilled water as a start point. Animals were kept under observation for one month.
Wounds were treated in the following manner; each dog (5 wounds) received all treatments. One wound treated with 0.9% saline solution as a control, second wound treated with propolis powder, third with MEBO, fourth with Panthenol and the last wound was treated with honey/cod liver oil mixture. Application of the medicament was carried out by direct topical application over the wound surface that was covered with piece of gauze soaked in the same medicament. The amount of the medicament measured about 5-6 ml (gm) for each wound surface. A protective bandage was applied to the wound surface. The frequency of changing dressing was changed, each two days for one week, then twice weekly for another week, and then once weekly till the end of the treatment or complete healing was achieved.
Evaluation of wound healing was based on; Clinical wound characters (including; degree of inflammation, wound exudation, wound infection, granulation tissue formation and epithelial tissue formation), Wound contraction percent (wound contraction % = W0 – WI / W0 X 100) and Histologic features for biopsies collected after 24 hrs, 3 days, 6 days and 16 days to determine the degree of cellular infiltration, collagen production, neovascularization and the thickness of epithelium over the wound.
The collected data in the present investigation are subjected to statistical analysis using One-Way ANOVA with Duncan’s multiple range tests.
The results of the experiment revealed that clinical wound characters following propolis powder showed complete granulation tissue formation and re-epethelization with absence of infection and exudation as compared with other medicaments. Mean reduction in wound length and width as well as mean wound contraction % showed significant increase after dressing with propolis powder.
The wound healing scores and parameters including re-epithelization, cellular infiltration, granulation tissue formation, vascularity and infection following histologic examination proved better in wounds treated with propolis powder and wounds treated with honey/cod liver oil mixture. Wounds treated with propolis powder provided moderately thick granulation tissue with more fibroblasts and collagen deposition and complete epithelial migration. There was no significant difference between the findings in wounds treated with either MEBO or Panthenol ointment.