الفهرس 
Review of literatureOnly 14 pages are availabe for public view
 |  | from | 181 |  |  |
| | | from | 181 | | |
Abstract
The organ samples were collected for isolation of infectious bronchitis virus. They were 50 sample from lung and 50 sample from trachea and 17 from oviduct from layer, Breeder and Broiler Breeder and Broiler chickens. These organs were prepared and inoculated in 9-11 day old embiyonated chicken eggs for three blind passages via the allantoic cavity using the commercial and specific pathogen free eggs. The dead embryos showed pathological changes (curling, dwarfing and thickness of the chorio allantoic membrane. The organs which gave positive pathological lesions on embryon and thickening of chorioallantoic membrane was 32.5 %.
The AGP test used for identification of the virus using reference IB serum. The result of positive AGP test was 25% in layer chickens 75 week of age, 12.5% in 55-week layer, 33% in 27-week layer, 33% in 20-week Breeder chickens, 33% in 10-week Broiler Breeder and 40% in 3-week Broiler chickens. The indirect immunofluoresence test for detection of Infectious bronchitis virus in empression smears of chorioallantoic membrane was done and the results was the presence of the virus in oviduct in 55-week layer chicken and in oviduct of 27-week layer chicken and in lung and trachea of 10-week broiler breeder chickens and in lung and trachea of 3-week broiler chickens. Negative results were obtained in oviduct of 75-week layer, and in lung and trachea of 27-week layer and 20 week breeder.
The effect of heat (56°c) and chloroforme 25% was used for identification of the isolated viruses. The results was that all the isolates was heat labile and sensitive for chloroform. The Hemagglutination-inhibition test was used for detection of antibodies against IBV using the phospholipase C-type-1 treated antigen. The results was the presence of HI antibodies in serum sample of 75-week layer chickens, 55-week layer, 27-week layer, 20-week Breeder, 10-week Broiler Breeder and 3-week Broiler chickens with a geometric mean of 7.0, 9.0, 6.6, 4.8, 6.2 and 4.8 alternatively. Also the AGP test used for detection of antibodies in serum samples. The results was the presence of AGP antibodies with a percentage of 6, 10, 13.3, 10, 10 and 12% in 75-week layer, 55-week layer, 27-week layer, 20-week Breeder, 10-week Broiler Breeder and 3-week Broiler in chickens alternatively. These samples were collected from Giza, Kalubia, Fayoum, and the Animal Health Research Institute. Also the ELISA test was used for detection antibodies to IBV. The results was rauged from 1132 titer to 93705. from the last results it was noticed that the infectious bronchitis is one of the viruses associated with respiratory affection in chickens. Also organ samples from 35-day Broiler chicken (lung-trachea) suffer from respiratory affection were collected for New-castle desease virus isolation. The samples were inoculated in 9-11 day-old embryonated chicken eggs via allantoic sac. A Heamagglutinating virus was isolated from dead and live embryos 5-7 days post inoculation. A Heamagglutination was 1:512 and Heamagglutination-inhibition against reference NDV positive antisera was positive for identification of the isolated virus. The Mean death time and Intracerebral pathogenicity index was done. The results was EID5o= 1083 / ml. And Mean death time (MDT) was 104 . The intracerebral pathogenicity index (ICPI) was 0.01. from these results the NDV was Lentogenic.
Also tracheal samples was collected containing diphtheritic lesions from laying chickens suffering from respiratory effections, and inoculated into 9-to12-day old embryonated chicken eggs via chorio-allantoic membrane (CAM). They examined for focal white pock lesions and generilised thickening of the CAMs.
The Histopathological examination results in that both ectoderm and endoderm were affected. Also the Histopathological examination of the CAM, lesions revealed eosinophilic intracytoplasmic inclusion bodies following staining with Haematoxylin and Eosin (H & E).
The Agar gel precipitation test, was conducted to identify the isolated virus using reference positive fowl pox antisera against the homogenized chorioallantoic membrane containing the pock lesions. A precipitatin line was obtained which proved that the virus was pox virus.