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العنوان
Characterizition of Salmonella Enteritidis and Plasmid Profiling =
المؤلف
El-Bahr, Helmy Mohammed Mohammed Hamed
هيئة الاعداد
باحث / حلمى محمد محمد حامد البحر
مشرف / محمد على عقيلة
مشرف / هانى فوزى اللقانى
مناقش / علاءالدين مصطفى حسين
مناقش / سامى عبدالسلام خليل
الموضوع
Microbiology- Bacteriology and Mycology.
تاريخ النشر
2013.
عدد الصفحات
74 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
24/12/2013
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - الميكروبيولوجيا
الفهرس
Only 14 pages are availabe for public view

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Abstract

Avian Salmonellosis is considered one of main factors that interfere with the success of poultry production among different species, Salmonella Enteritidis is considered the most important ecies causing infection among broiler chicken. This study was designed to isolate and characterize Enteritidis from broiler chickens and further performing plasmid profiling and antibiotic nsitivity;
125 cecal samples were collected from broiler chickens on tetrathionate broth and then bcultured on MacConkey agar. Pale colonies were identified as non-lactose fermenters and bcultured on nutrient slope agar and identified morphologically and biochemically.
The isolates that were catalase +ve, Oxidase —ye, Indole —ye, MR +ve, Citrate utilization +ve d Urease —ye were identified as Salmonella they were 27 isolates.
All the biochemically identified Salmonella isolates were sent to the central lab of ministry of aith for serotyping, among them only 5 were identified as Salmonella Enteritidis.
The 5 isolates were confirmed as S. Enteritidis by PCR using specific primers.
Plasmid profiling was carried on these isolates revealing the presence of different profile licating the presence of genotypic variation among these isolates and the presence of plasmid rrying gene responsible for resistance to antibiotic.
Antibiotic sensitivity on isolates was carried out; it was found that all isolates were highly ceptib1e to Enrofloxacin while resistant to Penicillin G, Amoxicillin and Tetracycline.