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Abstract Avian Salmonellosis is considered one of main factors that interfere with the success of poultry production among different species, Salmonella Enteritidis is considered the most important ecies causing infection among broiler chicken. This study was designed to isolate and characterize Enteritidis from broiler chickens and further performing plasmid profiling and antibiotic nsitivity; 125 cecal samples were collected from broiler chickens on tetrathionate broth and then bcultured on MacConkey agar. Pale colonies were identified as non-lactose fermenters and bcultured on nutrient slope agar and identified morphologically and biochemically. The isolates that were catalase +ve, Oxidase —ye, Indole —ye, MR +ve, Citrate utilization +ve d Urease —ye were identified as Salmonella they were 27 isolates. All the biochemically identified Salmonella isolates were sent to the central lab of ministry of aith for serotyping, among them only 5 were identified as Salmonella Enteritidis. The 5 isolates were confirmed as S. Enteritidis by PCR using specific primers. Plasmid profiling was carried on these isolates revealing the presence of different profile licating the presence of genotypic variation among these isolates and the presence of plasmid rrying gene responsible for resistance to antibiotic. Antibiotic sensitivity on isolates was carried out; it was found that all isolates were highly ceptib1e to Enrofloxacin while resistant to Penicillin G, Amoxicillin and Tetracycline. |