الفهرس 
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Abstract
Aggressive periodontitis is a complex inflammatory disease, which is a major cause of tooth loss. The risk for aggressive periodontitis is influenced by environmental and genetic factors. Matrix metalloproteinases gene polymorphisms may have an impact on the susceptibility to and progression of aggressive periodontitis. The matrix metalloproteinase-1 (MMP-1) is the key proteolytic enzyme capable of degradingfibrillar collagen, especially type I and III,which are the predominant types of interstitial collagenin the gingiva. The present study was conducted to investigate the association between the MMP1-519 A/G polymorphism and aggressive periodontitis in a sample of the egyptian population.
Sixty subjects were recruited for the study from the patient pool at the dental clinics of the oral medicine and periodontology department of the faculty of dentistry, Alexandria University. All subjects were non-smokers, Egyptians and with no systemic diseases. Subjects were divided into two main groups:
• Aggressive periodontitis patients group (n=30, 4 males and 26 females with mean age of 27.0 ± 5.88 years)
• Healthy controls group (n=30, 6 males and 24 females with mean age of 25.80 ± 2.30 years)
The diagnosis was made on the basis of standardized clinical and radiographic criteria.
Genomic DNA was obtained from peripheral blood leukocytes by the conventional “salting out” method. Genotyping of the promoter -519 A/G polymorphism of MMP1 gene was carried out using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) analysis. Genotype and allele frequency distributions among AP patients and controls were compared using chi squared (p<0.05) and Monte Carlo tests.
The frequency of the GG and AG genotypes were slightly higher in LAP patients (8.3% and 91.7%) compared to the GAP patients (5.6% and 88.9%) and AA genotype was only detected in GAP patients (5.6%) and not in LAP patients (0.0%), but also there was no statistical significant difference between the two groups (p=1.000). Also, the percentage of the G allele was higher in LAP patients (54.2%) than GAP patients (50%).
On the other hand, the percentage of the A allele in the GAP patients (50%) was slightly higher than the LAP patients (45.8%), but also there was not statistical significant difference between GAP and LAP patients regarding allele frequency (p=0.752).
The results of this study showed no statistical significant association between aggressive periodontitis and -519 A/G polymorphism of MMP1 gene. Also, the differences in allele and genotype frequency distribution between aggressive periodontitis patients and healthy controls were found to be not statistically significant (p=0.855 and 0.244 respectively).