الفهرس | Only 14 pages are availabe for public view |
Abstract One of the major problems of avian infectious bronchitis virus (IBV) is the frequent emergence of new variants. In the present study, 205 suspected farms from broilers and layers were collected during January to August 2012 from 19 governorates all over Egypt these farms showed respiratory signs. There were 130/205 (about 63.4%) of suspected farms were positive for IBV with real time RT-PCR. Thirteen samples were selected from 130 IBV positive samples. The samples were inoculated in specific pathogen free (SPF) embryos for three blind successive passages and the hypervariable region of spike protein (SP1) was amplified and sequenced to study the genetic diversity between viruses. The phylogenetic analysis was carried out between the selected 13 isolates and other IBV from the Middle East. Eleven out of the thirteen inoculums had close relationship with the Israeli variant (IS/885) reached up to 89.9%. The other two inoculums had close relationship with, CR/88121 and 4/91 viruses with identities 95% and 96%; respectively. |