الفهرس 
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Abstract
Environmental pollution is a one of the most deleterious agents to the biological life. Industrialization offered additional hazards to the environment surrounding man and animals. Because of a wide distribution of the heavy metals throughout the earth crust, in air, soil, and water, as well as the remarkable environmental pollution, these heavy metals represent global problems that are a growing threat to the environment. One of most important heavy metals is the cadmium chloride rampant in many industries such as cement plants and smoke, batteries and others.
The present investigation aimed to assess the biochemical effects of some natural products on cadmium induced toxicity in rats which are nigella sativa oil, lipoic acid and virgin olive oil.
The experiment was carried out on (50) male albino rats, these rats were divided randomly into five groups (each group consists of 10 rats only) as follows:
1) Control group: given physiological saline orally.
2) Cadmium (Cd) treated group : given 5mg\kg B.W\day CdCl2 dissolved in distilled water orally.
3) NSO and Cd treated group: given 5mg\kg b.w\day CdCl2+ Nigella sativa oil (NSO) at a dose of 2 ml \kg b.w\day orally.
4) LA and Cd treated group: given 5mg\kg b.w\day CdCl2 + Lipoic acid (100 mg\kg\day) orally.
5) OO and Cd treated group: given 5mg\kg b.w\day CdCl2+ Olive oil at a dose of 4 ml\kg b.w\day orally.
All treatments were administered daily by stomach tube for a period of 4 successive weeks, blood and tissue sample were collected at the end of the experimental period.
The effect on serum ALT, AST, total protein, albumin, creatinine, urea and acetyl cholinesterase were studied. In addition, GSH, MDA, CAT, SOD and DNA fragmentation % were measured in the tissue of liver, kidney and brain of rats involved in the present investigation.
Also the histopathological changes in the tissues of liver, kidney and brain of rats were evaluated.
Results were recorded in (10) tables and (30) figures. The obtained data were subjected to statistical analysis and revealed the following:
• Administration of cadmium chloride induced significant increase in each of serum ALT, AST, creatinine and urea. It also induced significant increase in CAT, SOD, MDA and DNA fragmentation % in the tissues of liver, kidney and brain of rats. In addition serum total protein and albumin concentrations and acetyl cholinesterase activity were significantly decreased. Also GSH content of liver, kidney and brain tissues homogenates of rats was significantly decreased.
• Our biochemical results were confirmed by histological investigations where many lesions were detected in liver kidneys and brain treated with cadmium.
• Administration of NSO, LA and OO with cadmium chloride showed a significant improvement in the measured biochemical parameters and histopathological pictures as compared with cadmium chloride treated group.
Conclusion and recommendations:
We conclude that the cadmium chloride induced biochemical and histopathological changes in liver , kidney and brain of rats, which were improved by administration of nigella sativa oil, lipoic acid and olive oil.
We advised to use both of NSO, LA and olive oil in the prevention and treatment of cadmium chloride toxicity, especially olive oil which exhibited more significant effect.
It is also advisable to give NSO, LA and olive oil to workers in factories that their products contain the contaminated particles with cadmium chloride, especially cement factories.
Further investigations concerning the recommended dose and duration in human beings are required to establish the safest dose.