الفهرس 
Introductionيوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
The present work was planned to study some biochemical alterations in the blood, tissues as well as histopathological changes in mice infected with S. mansoni before and after oviposition.
For performing the present work, 80 Swiss albino mice one month old, weighting 20-25 gm and free from parasitic infection by examining their stool for 7 successive days, were used. They were kept under hygienic conditions and fed on both barly and milk ad libitum allover the experiment to avoid any possible effect of malnutrition. The mice infected by subcutaneous injection with 60 S. mansoni cercariae for each mouse.
The blood samples were collected from each mouse in each group after sacrificing in a clean dry tube containing heparin as anticoagulant. The whole blood samples were served for measuring GSH, SOD, Hb and G6PDH. The remaining of each blood sample from each mouse in each group were centrifuged at 3000 rpm for 15 minutes. The obtained non-hemolyzed plasma were used for measuring plasma iron and
ceruloplasmin.
The results obtained showed that;
A significant decrease in the level of blood reduced glutathione in mice infected with S. mansoni before oviposition. This decrease became highly significant after oviposition as compared with control noninfected groups. The hepatic reduced glutathione showed a highly significant increase in mice infected with S. mansoni before and after oviposition as compared with control groups.
A highly significant decrease in the activity of glucose-6-phosphate dehydrogenase in blood of infected mice with S. mansoni before and after oviposition as compared with the control (noninfected) groups. The hepatic glucose-6-phosphate dehydrogenase activity revealed highly significant increase in infected mice with S. mansoni before and after oviposition as compared with the control (non-infected) groups.
A highly significant increase in blood superoxide dismutase in infected mice before and after oviposition were observed as compared with the control (non-infected) groups. Also, the hepatic superoxide dismutase showed highly significant increase in infected mice before and after oviposition.
A highly significant decrease in hemoglobin level in infected mice with S. mansoni before and after oviposition was recorded as compared with the control (non-infected) groups.
A significant increase in plasma iron level in infected mice before oviposition whereas a highly significant decrease in plasma iron level was observed in infected mice as compared with the control (non-infected) group.
Microscopically, it showed the presence of adult schistosomes in the portal vein. The hepatic parenchyma showing scattered numerous eggs (granulomata) surrounded by macrophages, lymphocytes, eosinophiles and few giant cells. Small areas of coagulative necrosis particularly adjacent to to granulomata were present. The portal tracts and hepatic sinusoides were infiltrated with macrophage’s, eosinophiles and lymphocytes. Dark brown bilharzial pigments were seen in the kupffer cells and inside the macrophages around the egg granulomata.