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العنوان
Studies On Bioproduction Of Gibberellic Acid from Fungi /
المؤلف
Sleem, Doaa Abd El Monem Emam.
هيئة الاعداد
باحث / دعاء عبد المنعم إمام سليم
مشرف / محمود محمد هزاع
مشرف / سهام محمد شاش
مناقش / هشام محمود سويلم
مناقش / ناجى حليم عزيز
الموضوع
Fungi.
تاريخ النشر
2013.
عدد الصفحات
163 p. ؛
اللغة
العربية
الدرجة
الدكتوراه
التخصص
علم الأحياء الدقيقة
تاريخ الإجازة
1/1/2013
مكان الإجازة
جامعة بنها - كلية العلوم - الميكروبيولوجي
الفهرس
يوجد فقط 14 صفحة متاحة للعرض العام

from 185

from 185

المستخلص

Abstract Gibberellic acid is a natural plant growth hormone which is gaining much more attention all over the world due to its effective use in agriculture and brewing industry. At present gibberellic acid is produced throughout the world by fermentation technique using the fungus Gibberella fujikuroi (recently named Fusarium moniliforme). The aim of the current study is the isolation of local F. moniliforme isolate have the ability to produce gibberellic acid on specific production media. The submerged fermentation technique for the production of gibberellic acid is influenced to a great extent by a variety of physical factors (incubation time, temperature, pH, agitation speed) also, gibberellic acid production by F. moniliforme depends upon the nature and concentrations of carbon and nitrogen sources. The optimization of these factors is prerequisite for the development of commercial process. The addition of some elements in a significant quantities to the production media stimulate gibberellic acid production. The use of seed culture inocula (24 h) age at rate of (2% v/v) also enhance the production. Working volume 50 ml in 250 ml Erlenmeyer flask was found to be the best volume for the production. Low doses of gamma radiation (0.5 kGy) stimulate gibberellic acid production and microbial growth by the local F. moniliforme isolate. Immobilized cell fermentation technique had also been developed as an alternative to obtain higher yield of gibberellic acid. Milk permeate (cheap dairy by- product) was found suitable to used as main production medium for gibberellic acid production by the fungus under investigation. The influence of gibberellic acid on enhancement growth of Aspergillus niger and chitosan production was also studied, the addition of 2 mg/l of gibberellic acid to chitosan production medium stimulate its production in comparison with media without gibberellic acid.