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Abstract The present investigation was designed to study the bacteriological quality of 100 samples comprised four species of marine water fish namely (Sardine, Mullus spp. (Morgan), Pargus spp. (Barbone) and Chrysophyres (Dinisj) (25 of each) which were collected from commercial fish markets in Alexandria governorate over a period of 6 months. So, this study was designed to throw a light on the hygienic quality of four common marine fishes sold in these markets. Regarding to the microbiological status ofthe examined fishes,( Sardine, Mullus spp., Pargus spp. and Chrysophyres) the mean Total Bacterial counts values were 3.95 x 106 + 1.26 X 106,3.67 X 106+ 0.798 X 106, 3.82x 106+ 0.786 x 106 and 2.l6x 106+ 0.587 x 106 CFU/g ,respectively, the mean total Enterobacteriaceae counts values were 6.57 x104+2.32x104,3.35x1OS +0.731 x 105 ,3.18x10s +0.752xlOs and 1.76 x 105 + 0.522 x 105CFU/g ,respectively, the mean total coliforms counts values were 8.09 x 103 + 2.59 X 103 , 1.24 x 105 + 0.379 x 105, 1.32 x 105 + 0.418 x 10’ and 4.07 x 104+ 1.81 x 104CFU/g ,respectively, the mean totaltoterococci counts values were 6.04 x 103+ 2.81 X 103,2.05 X 104 + 0.• 10\ 1.41 X 104+ 0.43 X 104 and 2.41 X 104 + 0.596 X 104 CFU/g ,resp e ”.ely, While the mean total staphylococci counts values were 2.82 x 11’+ 0.723 x 103 , 3.%x loJ+ 0.907 x 103 , 3.18 x io’ + 0.811 x io’ ••• S.28 x 103+ 1.41 x loJ CFU/g ,respectively, The incidences of different organisms in the examined fish species namely, Sardine, Mullus spp., Pargus spp. and Chrysophyres for total Enterobacteriaceae were 84, 92,100 and 92 % respectively, for total coliforms 72, 92, 100 and 92 % respectively, for total Enterococci 84, 100, 100 and 100 % respectively and for total Staphylococci 92, 100, 100 and 100 % respectively for the aforementioned fish species. Correlation between the microbiological load and fish species results in non statistically significant differences in the mean total bacterial counts and total Staphylococci counts between the four aforementioned examined fish species. While, there are correlation between the microbiological load and fish species results in statistically significant differences in the mean total Enterobacteriaceae counts between Sardine species (in one arm) and Mullus spp. and Pargus spp. (in the other arm). Also, Correlation between the microbiological load and fish species results in there was no statistical significant difference between the mean of total coliforms counts of Sardine and Chrysophyres, and also there is no statistical signific.t difference between the mean of total coliforms counts of Mull us W., Pargus spp. and Chrysophyres. But, there was a statistical signifiMnt difference between the mean of total coliforms counts of Sardine (in one arm) and both Mullus spp. and Pargus spp. (in other arm). And, Correlation bet IIm the microbiological load and fish species results in there was no statillkal significant difference of means Enterococci counts value between Sardine and Pargus spp. but it was present between Sardine (in one arm) and both Mullus spp. and Chrysophyres (in the other arm) . Also, there were no statistical significant difference of means Enterococci counts values between Mullus spp., Pargus spp., and Chrysophyres. Regarding to the mycological evaluation of the examined fishes revealed that the mean total mould counts values of,( Sardine, Mullus spp., Pargus spp. and Chrysophyres) were 3.42 + 0.596 x 102, 3.25 + 0.589 X 102,2.75 +0.554 x 102 and 3.75 + 0.528 x 102CFU/g ,respectively, and for yeast 3.52 + 0.809 x 102, 3.39+ 0.58 X 102,3.12 + 0.63 x 102 and 5.61 + 1.57 x 102 CFU/g ,respectively. Correlation between the mycological load and fish species results in non statistically significant differences in the mean total mould and yeast counts between the four aforementioned examined fish species. The present investigation was designed to study the bacteriological quality of 100 samples comprised four species of marine water fish namely (Sardine, Mullus spp. (Morgan), Pargus spp. (Barbone) and Chrysophyres (Dinisj) (25 of each) which were collected from commercial fish markets in Alexandria governorate over a period of 6 months. So, this study was designed to throw a light on the hygienic quality of four common marine fishes sold in these markets. Regarding to the microbiological status ofthe examined fishes,( Sardine, Mullus spp., Pargus spp. and Chrysophyres) the mean Total Bacterial counts values were 3.95 x 106 + 1.26 X 106,3.67 X 106+ 0.798 X 106, 3.82x 106+ 0.786 x 106 and 2.l6x 106+ 0.587 x 106 CFU/g ,respectively, the mean total Enterobacteriaceae counts values were 6.57 x104+2.32x104,3.35x1OS +0.731 x 105 ,3.18x10s +0.752xlOs and 1.76 x 105 + 0.522 x 105CFU/g ,respectively, the mean total coliforms counts values were 8.09 x 103 + 2.59 X 103 , 1.24 x 105 + 0.379 x 105, 1.32 x 105 + 0.418 x 10’ and 4.07 x 104+ 1.81 x 104CFU/g ,respectively, the mean totaltoterococci counts values were 6.04 x 103+ 2.81 X 103,2.05 X 104 + 0.• 10\ 1.41 X 104+ 0.43 X 104 and 2.41 X 104 + 0.596 X 104 CFU/g ,resp e ”.ely, While the mean total staphylococci counts values were 2.82 x 11’+ 0.723 x 103 , 3.%x loJ+ 0.907 x 103 , 3.18 x io’ + 0.811 x io’ ••• S.28 x 103+ 1.41 x loJ CFU/g ,respectively, The incidences of different organisms in the examined fish species namely, Sardine, Mullus spp., Pargus spp. and Chrysophyres for total Enterobacteriaceae were 84, 92,100 and 92 % respectively, for total coliforms 72, 92, 100 and 92 % respectively, for total Enterococci 84, 100, 100 and 100 % respectively and for total Staphylococci 92, 100, 100 and 100 % respectively for the aforementioned fish species. Correlation between the microbiological load and fish species results in non statistically significant differences in the mean total bacterial counts and total Staphylococci counts between the four aforementioned examined fish species. While, there are correlation between the microbiological load and fish species results in statistically significant differences in the mean total Enterobacteriaceae counts between Sardine species (in one arm) and Mullus spp. and Pargus spp. (in the other arm). Also, Correlation between the microbiological load and fish species results in there was no statistical significant difference between the mean of total coliforms counts of Sardine and Chrysophyres, and also there is no statistical signific.t difference between the mean of total coliforms counts of Mull us W., Pargus spp. and Chrysophyres. But, there was a statistical signifiMnt difference between the mean of total coliforms counts of Sardine (in one arm) and both Mullus spp. and Pargus spp. (in other arm). And, Correlation bet IIm the microbiological load and fish species results in there was no statillkal significant difference of means Enterococci counts value between Sardine and Pargus spp. but it was present between Sardine (in one arm) and both Mullus spp. and Chrysophyres (in the other arm) . Also, there were no statistical significant difference of means Enterococci counts values between Mullus spp., Pargus spp., and Chrysophyres. Regarding to the mycological evaluation of the examined fishes revealed that the mean total mould counts values of,( Sardine, Mullus spp., Pargus spp. and Chrysophyres) were 3.42 + 0.596 x 102, 3.25 + 0.589 X 102,2.75 +0.554 x 102 and 3.75 + 0.528 x 102CFU/g ,respectively, and for yeast 3.52 + 0.809 x 102, 3.39+ 0.58 X 102,3.12 + 0.63 x 102 and 5.61 + 1.57 x 102 CFU/g ,respectively. Correlation between the mycological load and fish species results in non statistically significant differences in the mean total mould and yeast counts between the four aforementioned examined fish species. The public health significance of the isolated microorganisms were discussed and the suggestive measures to protect the consumer and produce high quality fish were outlined.discussed and the suggestive measures to protect the consumer and produce high quality fish were outlined. |