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Abstract The present investigation was conducted at the Experimental Farm Sers El-Laian Agricultural Research Station, Agricultural Research Center, Egypt, to investigate the effect of plant densities, organic and biofertilization on growth, abscission, nodulation, yield components and some technological characteristics of faba bean (Vicia faba L.) cultivars during the successive winter seasons of 2009/2010 and 2010/2011. The experiment included fifty four treatments in each growing season which were the combination of three plant densities, three cultivars and six fertilization systems which are as follows: A- Plant densities. 1- 70,000 plant/fed 2- 105,000 plant/fed 3- 140,000 plant/fed B- Cultivars. 1- Giza 3 2- Giza 716 3- Nubaria 1 C- Fertilization systems. 1- Untreated (Control). 2- Biofertilizers (mixed inocula of Rhizobium, Azotobacter, Azospirillum and Bacillus spp.). 3- Compost at a rate of 4 ton/fed. 4- Compost at a rate of 6 ton/fed. 5- Compost at a rate of 4 ton/fed + biofertilizers. 6- Compost at a rate of 6 ton/fed + biofertilizers. The Treatments were arranged at random in a split split plot design with three replications. The main plots were allocated for plant densities. However, the sub-plots were devoted for the cultivars and the sub-sub plots were occupied by fertilization systems. The obtained results could be summarized as follows: I- Growth analysis 1- Differences among the three tested plant densities were found among characters studied. Plant density of 70,000 plant/fed surpassed the other densities in numbers of branches and leaves /plant, leaf area and stem, leaves, pods and total dry weights/plant. However, the density of 140,000 plant/fed exhibited greatest plant height in both seasons. 2- Differences among the three tested faba bean cultivars were observed among characters studied. In this respect, Giza 3 surpassed the other cultivars in plant height, while Nubaria 1 had substantial increases in numbers of branches and leaves/plant, leaf area/plant and leaves dry weight /plant. However, Giza 716 exhibited greatest stem, pods and total dry weights/plant in both seasons. 3- Data of the growth characters indicated that application of 6 ton compost + biofertilizers gave the highest values of plant height, numbers of branches and leaves/plant, leaf area per plant, dry weight of different organs /plant compared to the other fertilization systems. However, application of 4 ton compost + biofertilizers ranked as a second in this respect during all growth stages in both seasons. 4- The interaction between densities and cultivars on number of branches and pods dry weight/plant were statistically significant during both seasons. It clear that Nubaria 1 possessed their highest mean values of number of branches /plant when the plants were planted under density of 70000 plant, while Giza 716 cultivar had the highest values of pods dry weight when the plants were under density of 70000 plant. 5- Concerning the interaction between densities and fertilization, the highest mean values of stem, leaves, pods and total dry weights /plant were obtained from plant density (70,000 plant / fed) and fertilization with 6 ton com +bio treatment in the two seasons. 6- The interaction showed that Giza 3 was produced the tallest plants when fertilized with 6 ton compost+bio. Meanwhile, Nubaria 1 possessed their higher mean values of leaf area and leaves dry weight/plant when the plants were fertilized with 4 or 6 ton compost+bio. On the other side, maximum values of stem, pods and total dry matter were obtained from sowing Giza 716 and fertilized with 6 ton compost+bio in both seasons. 7- The interactions between the three factors were found to be significant for leaf area and dry weight of different organs of plant in both seasons. Nubaria 1 produced the highest values of leaf area and leaves dry weight when plants were sowed under the density of 70000 plant/fed and fertilized with 4 or 6 ton compost + bio. However, the highest values of stem, pods and total dry weight were obtained by sowing Giza 716 under density of 70000 plant /fed and fertilized with 6 ton compost+bio. II- Numbers and abscission of flowers and pods 1- The results showed that density of 70000 plant/fed recorded the highest number of flowers and setting pods/plant, while the dense density produced the lowest ones in both seasons. On the other hand, the lowest density produced lower percentages of flowers, pods and total abscissions per plant than mid and dense densities in both seasons. 2- Giza 716 cultivar produced the highest number of flowers/plant while Nubaria 1 produced the lowest one in both seasons. However, Giza 3 cultivar surpassed the other tested cultivars in number of setting pods/plant and produced the lowest percentages of flowers, setting pods and total abscissions per plant than other cultivars in both seasons. 3- The highest number of flowers and setting pods/plant were obtained by the application of 6 ton compost+bio followed by 4 ton compost+bio treatment. Also, application of 6 ton compost+bio significantly reduced the abscission percentages compared to other fertilization systems. 4- The interaction between densities and cultivars indicated that sowing Giza 716 cultivar under the plant density of 70,000 or 105,000 plant/fed produced the highest number of flowers/plant without significant differences in the both seasons. However, the highest number of setting pods /plant and lowest flower, setting pods and total abscission% were obtained by sowing Giza 3 under density of 70,000 plant/fed. 5- The interaction between densities and fertilization showed that sowing plants under the density of 105,000 plant/fed and fertilized with 6 ton compost+bio surpassed the other treatments in number of flowers/plant. However, the maximum number of setting pods and lowest values of abscission % were recorded by fertilized plants with 6 ton compost +bio under the density of 70,000 plant in the both seasons. 6- The interaction between cultivars and fertilization systems showed that the highest values of numbers of flowers and setting pods/plant were obtained from fertilized Giza 3 cultivar with 6 ton compost + bio, while the lowest ones obtained from unfertilized Nubaria 1 plants. On the other hand, the lowest values of abscission % were recorded mostly when fertilized Giza 3 plants with 4 ton compost + bio or 6 ton compost + bio in both seasons. 7- The interaction among the three factors showed that planting Giza 3 under the density of 70,000 plant/fed and fertilized with 6 ton compost + bio significantly increased number of setting pods, while reduced the values of flowers, setting pods and total abscission % compared to other cultivars under other densities and fertilization systems. III- Nodulation and nitrogenase activity 1- The data demonstrate that were significant differences among the tested densities for all characters studied in both seasons. The highest nodules number and weight/plant and nitrogenase activity were obtained by density of 70,000 plant/fed followed by density of 105,000 plant/fed during all growth stages in the two seasons. 2- Nubaria 1 cultivar surpassed the other cultivars in nodulation and nitrogenase activity, while Giza 3 ranked inferior in this respect during all growth stages in the two seasons. 3- Application of compost, biofertilizers and their mixture caused an increase in nodulation and nitrogenase activity compared to unfertilized plants, where applying 6 ton compost+bio produced the highest values. 4- The data of the interaction between densities and cultivars showed that maximum nodules number and weight/plant and nitrogenase activity were obtained when sowed Nubaria 1 cultivar under the density of 70,000 plant/fed. However, the lowest significant values were obtained when sowing Giza 3 cultivar under the density of 140000 plant/fed. 5- The interaction between the tested densities and fertilization systems was significant for nodulation and nitrogenase activity. The data indicated that the sowing plants under the density of 70,000 plant/fed and fertilized with 6 ton compost+bio produced the highest significant values. However, the lowest significant values were obtained when the plants were cultivated under the density of 140,000 plant/fed and unfertilized with any fertilization systems. 6- Interaction data clear that treatment of fertilized Nubaria 1 plants with 6 ton compost+bio produced the highest values of nodules number and dry weight /plant and nitrogenase activity, while the lowest values were obtained by unfertilized Giza 3 plants during both seasons. 7- Data of the interaction among the three factors showed that planting Nubaria 1 cultivar under the density of 70,000 plant/fed and fertilized plants with 6 ton compost+bio was more the effective treatment for increasing nodulation and nitrogenase compared to other treatments. IV-Yield and yield components 1- The results denoted that yield and yield components were significantly differed by tested plant densities. Results indicated that the highest yield components of plant were resulted from the lowest plant density, while the maximum seed, straw and biological yields/fed were recorded by the dense density in both seasons. 2- Data showed that Giza 3 cultivar produced the highest number of pods/plant and seed yield per plant and fed, while Nubaria 1 cultivar surpassed the other cultivars in number of seeds/pod, 100-seed weight, seed weight/pod and straw and biological yields/fed in both seasons. 3- Application of the compost and biofertilization especially the highest mixture caused a significant increase in all yield and its components characters compared to unfertilized plants in both seasons. 4- In both seasons, the interaction between densities and cultivars indicated that sowing Giza 3 cultivar under the lowest density produced the highest number of pods/plant and seed yield/plant. However, Nubaria 1 under the same density recorded the highest number of seeds /pod and 100-seed weight. The maximum yield of seed/fed was obtained by sowing Giza 3 under dense density, while straw and biological yields were obtained by Nubaria 1 under the dense density. 5- Interaction effect showed that the highest yield components of plant were resulted from the lowest plant density and fertilized plants with 6 ton compost+bio, while the maximum seed, straw and biological yields/fed were recorded by the dense density and fertilized plants with 6 ton compost+bio in both seasons. 6- Concerning interaction between cultivars and fertilization, fertilized Giza 3 plants with 6 ton compost+bio seemed to be the most effective treatment on increasing the number of pods/plant and seed yield per plant and fed. However, fertilized Nubaria 1 plants with 6 ton compost+bio produced the highest values of number of seeds/pod, 100- seed weight, seed weight/pod and straw and biological yields/fed. 7- In both seasons, the triple interactions showed that Giza 3 cultivar possessed their highest values when fertilized with 6 ton compost+bio under the density of 70,000 plant/fed for number of pods and seed yield /plant characters and under the density of 140,000 plant/fed for seed yield/fed. However, Nubaria 1 cultivar produced their highest values when fertilized with 6 ton compost+bio under the density of 70,000 plant/fed for number of seeds /pod and 100- seed weight and under the density of 140,000 plant/fed for straw and biological yields/fed. V- Chemical analysis of plant dry matter and seeds: 1- The highest NPK percentage and uptake in dry matter and seeds were obtained with the lowest plant density. However, the lowest values obtained by increasing density up to 140,000 plant/fed. 2- Nubaria 1 cultivar was the most superior one for NPK% in dry matter and seeds than the other two cultivars, while Giza 716 and Giza 3 cultivars exhibited maximum values of NPK uptake in dry matter and seeds, respectively in both seasons. 3- Fertilization plants with compost, biofertilizers and their mixture caused a significant increase for NPK % and uptake in dry matter and seeds in favor of 6 ton compost+bio compared to unfertilized plants. 4- In both seasons, the interaction between the densities and cultivars had no significant effects on NPK% and uptake in dry matter and seeds. 5- The interaction between the densities and fertilization did not reach the level of significant for NPK% and uptake in both seasons. 6- The NPK percentage and uptake were not significantly affected by the interaction between cultivars and fertilization in both seasons. 7- The NPK% and uptake in dry matter and seeds were not significantly affected by the interaction among the three factors during both seasons. VI- Technological suitability of seeds 1- The highest plant density gave the highest mean values of TSS and imbibed water % in the two seasons. Meanwhile the density of 105,000 and 140,000 plant/fed produced the highest values of stewing % without significant difference between the two densities. 2- It is obvious that the Giza 3 cultivar surpassed the other two cultivars in TSS and imbibed water % of faba bean seeds. However, Nubaria 1 produced the highest stewing values. 3- Fertilization plants with 6 ton compost + bio followed by 4 ton compost + bio produced the highest values of TSS, imbibed water and stewing% than that obtained by the rest treatments. One the contrary, the lowest values was obtained by unfertilized plants. 4- The interaction between the densities and cultivars had no significant effects on seed technological characters. 5- The interaction between the densities and fertilization did not reach the level of significant for seed technological characters. 6- The interaction between the cultivars and fertilization showed that the highest values of TSS and imbibed water % were recorded when fertilized Giza 3 plants with 6 ton compost + bio. However, fertilized Nubaria 1 and Giza 716 cultivars with 6 ton compost + bio gave the highest values of stewing % without significant between them. 7- Results indicated that seed technological characters were not significantly affected by the interaction among the three factors in both seasons. |