الفهرس 
Introduction
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Abstract
Pulpy kidney disease caused by C. perfringens type D which
secret epsilon toxin cause sudden death, diarrhea and nervous
manifestation result in great economical and sanitary importance for
cattle, sheep and goat. The vaccination against pulpy kidney disease
depends mainly on epsilon toxoid. Some factors affecting the release
of epsilon toxin were studied. It was found that using TGY media at
pH 8.5 at 42ºC was optimal for bacterial growth and at 33ºC for
maximal toxin yield with incubation for 48 hours. It was also found
that the combination between trypsin and traces of chymotrypsin at
pH 7.2 for 45 minutes incubation at 37ºC was economical and more
effective for activation of epsilon protoxin. The using of chitosan as
an adjuvant for the vaccine give higher immune response in
vaccinated animals than that vaccinated with alum precipitated one.
Finally the results revealed great variation in immune response
between prepared vaccines in comparison to the current routinely
used vaccine. So we recommend replacement of formulation of
routine vaccine by the prepared one.