الفهرس 
CANDIDAOnly 14 pages are availabe for public view
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Abstract
total number of sixty samples of oral swabs were collected. Forty patient samples were collected from out-patients of Pediateric department in Ain Shams University Hospital that were clinically diagnosed as Thalassemia major. Twenty control samples were collected from age and sex matched healthy subjects.
Oral swab samples were sent immediately to the medical microbiology and immunity department in Fuclty of Medicine Ain Shams University and cultured as soon as possible on CHROM agar medium at 37°C for 48 - 72 hours.
The produced colonies were examined for colonial morphologies including size, shape, surface and pigment production. In addition, growths were examined both microscopically and biochemically followed by subculture on Mueller-Hinton agar media at 37°C for 48 - 72 hours for confirmation and antifungal susceptibility tests.
Germ tube test was done for all colonies grown on sabrourand’s agar medium to differntitate between Candida albicans and non albicans Candida species and compare the results with those obtained from CHROM agar.
Regarding the organisms isolated by the culture, C. albicans, was isolated from 69.2% of case. 30.8% were non C. albicans. (23% C. tropicalis and 7.8% C. krusei).
The results showed that history of splenectomy, and high serum ferritin levels were significant factors associated with Candidal colonization among thalassemic patients.
CHROM agar media succeeded in detecting all the Candida species that was detected by conventional methods. All strains developed the expected color as confirmed by API.
It can be concluded that CHROM agar media is a good alternative for conventional cultures for more rapid and easier identification of Candida species And supports the use of CHROMagar Candida for the rapid identification of Candida species directly from clinical specimens in resource-limited settings, which could be very helpful in developing appropriate therapeutic strategy and management of patients.
API is good test but expensive so can’t be used as routine method.
Antifungal susceptibility test was done to all positive isolates by disc diffusion method for three drugs (Fluconazole, Vericonazole and Nystatin) and the results showed that (13.8%) of all isolates were resistant to fluoconazole, (17.3%) to voriconazole and (89.7%) to nystatin.